| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Phospho-AKT (pY326) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the phospho-AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-AKT antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P31749 |
| Purity: |
Antigen affinity |
| Description: |
AKT1 and the related AKT2 are activated by platelet-derived growth factor, In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival, It was shown that the activation occurs through phosphatidylinositol 3-kinase, Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1, which then phosphorylates and inactivates components of the apoptotic machinery, The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts |
| Immunogen: |
This phospho-AKT antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY326 of human AKT |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-AKT antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Phospho-AKT (pY326) |
| Short name: |
Phospho-AKT Antibody (pY326) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
phosphorilated-AKT (Antibody to) (pY326) |
| Alternative technique: |
antibodies |
| Identity: |
391 |
| Gene: |
AKT1 |
More about : AKT1 |
| Long gene name: |
AKT serine/threonine kinase 1 |
| Synonyms gene name: |
v-akt murine thymoma viral oncogene homolog 1 |
| Synonyms: |
RAC PKB PRKBA AKT |
| Locus: |
14q32, 33 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
M63167 |
| Entrez gene record: |
207 |
| RefSeq identity: |
NM_005163 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000170795 |
| Locus Specific Databases: |
LRG_721 |