| Category: |
Antibody |
| Concentration: |
09% sodium azide, 4 with 0, In 1X PBS pH 7 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Cyclin D1 (CCND1) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IF, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the Cyclin D1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Cyclin D1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P24385 |
| Purity: |
Antigen affinity |
| Description: |
Also substrate for SMAD3, CyclinD-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals, Hypophosphorylates RB1 in early G(1) phase, Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase, [UniProt], phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity, CCND1 is the regulatory component of the Cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition |
| Immunogen: |
A portion of amino acids 68-97 from the human protein was used as the immunogen for this Cyclin D1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Cyclin D1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Cyclin D1 (CCND1) |
| Short name: |
Cyclin D1 Antibody (CCND1) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Cyclin D1 (Antibody to) (cyclin D1) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BCL1 and D11S287E and PRAD1 and U21B31, CCND1 and IDBG-61868 and ENSG00000110092 and 595, CCND1 and IDBG-645012 and ENSBTAG00000017514 and 524530, Ccnd1 and IDBG-212973 and ENSMUSG00000070348 and 12443, DNA-templated and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0007049 and cell cycle and biological process this GO :0007219 and Notch signaling pathway and biological process this GO :0007595 and lactation and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0010033 and response to organic substance and biological process this GO :0010039 and response to iron ion and biological process this GO :0010165 and response to X-ray and biological process this GO :0010243 and response to organonitrogen compound and biological process this GO :0010971 and positive regulation of G2/M transition of mitotic cell cycle and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0016020 and membrane and cellular component this GO :0016055 and Wnt signaling pathway and biological process this GO :0016301 and kinase activity and molecular function this GO :0016538 and cyclin-dependent protein serine/threonine kinase regulator activity and molecular function this GO :0017053 and transcriptional repressor complex and cellular component this GO :0019899 and enzyme binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0030178 and negative regulation of Wnt signaling pathway and biological process this GO :0030857 and negative regulation of epithelial cell differentiation and biological process this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0031100 and organ regeneration and biological process this GO :0031571 and mitotic G1 DNA damage checkpoint and biological process this GO :0032026 and response to magnesium ion and biological process this GO :0032403 and protein complex binding and molecular function this GO :0033197 and response to vitamin E and biological process this GO :0033327 and Leydig cell differentiation and biological process this GO :0033598 and mammary gland epithelial cell proliferation and biological process this GO :0033601 and positive regulation of mammary gland epithelial cell proliferation and biological process this GO :0042493 and response to drug and biological process this GO :0042826 and histone deacetylase binding and molecular function this GO :0043627 and response to estrogen and biological process this GO :0045444 and fat cell differentiation and biological process this GO :0045471 and response to ethanol and biological process this GO :0045737 and positive regulation of cyclin-dependent protein serine/threonine kinase activity and biological process this GO :0048545 and response to steroid hormone and biological process this GO :0051301 and cell division and biological process this GO :0051384 and response to glucocorticoid and biological process this GO :0051412 and response to corticosterone and biological process this GO :0051592 and response to calcium ion and biological process this GO :0051726 and regulation of cell cycle and biological process this GO :0060070 and canonical Wnt signaling pathway and biological process this GO :0060749 and mammary gland alveolus development and biological process this GO :0070064 and proline-rich region binding and molecular function this GO :0070141 and response to UV-A and biological process this GO :0071157 and negative regulation of cell cycle arrest and biological process this GO :0071310 and cellular response to organic substance and biological process this GO :2000045 and regulation of G1/S transition of mitotic cell cycle and biological process, nuclei, proline-rich region binding, this GO :0000079 and regulation of cyclin-dependent protein serine/threonine kinase activity and biological process this GO :0000082 and G1/S transition of mitotic cell cycle and biological process this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000278 and mitotic cell cycle and biological process this GO :0000307 and cyclin-dependent protein kinase holoenzyme complex and cellular component this GO :0000320 and re-entry into mitotic cell cycle and biological process this GO :0001889 and liver development and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0003714 and transcription corepressor activity and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005923 and tight junction and cellular component this GO :0006351 and transcription, this GO :0003714 : transcription corepressor activity, this GO :0003714 : transcription corepressor activity and also this GO :0004672 : protein kinase activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0016301 : kinase activity and also this GO :0016538 : cyclin-dependent protein serine/threonine kinase regulator activity and also this GO :0019899 : enzyme binding and also this GO :0019901 : protein kinase binding and also this GO :0032403 : protein complex binding and also this GO :0042826 : histone deacetylase binding and also this GO :0070064 : proline-rich region binding, this GO :0004672 : protein kinase activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0016301 : kinase activity, this GO :0016538 : cyclin-dependent protein serine/threonine kinase regulator activity, this GO :0019899 : enzyme binding, this GO :0019901 : protein kinase binding, this GO :0032403 : protein complex binding, this GO :0042826 : histone deacetylase binding, this GO :0070064 : proline-rich region binding, cyclin D1 |
| Identity: |
1582 |
| Gene: |
CCND1 |
More about : CCND1 |
| Long gene name: |
cyclin D1 |
| Synonyms gene: |
BCL1 D11S287E PRAD1 |
| Synonyms gene name: |
cyclin D1 (PRAD1: parathyroid adenomatosis 1) |
| Synonyms: |
U21B31 |
| Synonyms name: |
parathyroid adenomatosis 1 B-cell CLL/lymphoma 1 G1/S-specific cyclin D1 |
| Locus: |
11q13, 3 |
| Discovery year: |
1991-06-06 |
| GenBank acession: |
Z23022 |
| Entrez gene record: |
595 |
| Pubmed identfication: |
1826542 1833066 |
| RefSeq identity: |
NM_053056 |
| Classification: |
Cyclins |
| Havana BLAST/BLAT: |
OTTHUMG00000167877 |
| Locus Specific Databases: |
LRG_990 |