| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
UCH37 (UCHL5) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the UCH37 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This UCH37 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9Y5K5 |
| Purity: |
Purified |
| Description: |
(1989) purified the predominant isozyme, All 4 were thiol proteases and had high-affinity binding sites for ubiquitin, By screening a human B-cell expression library with the antibodies, Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus, Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1, There are 2 distinct families of DUBs, UCHL3, Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, Wilkinson et al, and raised antibodies against it, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers, the authors isolated cDNAs encoding human UCHL3, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs), Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes |
| Immunogen: |
A portion of amino acids 56-87 from the human protein was used as the immunogen for this UCH37 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the UCH37 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
UCH37 (UCHL5) |
| Short name: |
UCH37 Antibody (UCHL5) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
UCH37 (Antibody to) (ubiquitin carboxyl-terminal hydrolase L5) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0008242 and omega peptidase activity and molecular function this GO :0010951 and negative regulation of endopeptidase activity and biological process this GO :0016579 and protein deubiquitination and biological process this GO :0021670 and lateral ventricle development and biological process this GO :0030512 and negative regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030901 and midbrain development and biological process this GO :0031011 and Ino80 complex and cellular component this GO :0044822 and poly(A) RNA binding and molecular function this GO :0048853 and forebrain morphogenesis and biological process this GO :0061136 and regulation of proteasomal protein catabolic process and biological process this GO :0070628 and proteasome binding and molecular function, UCHL5 and IDBG-105454 and ENSG00000116750 and 51377, UCHL5 and IDBG-629660 and ENSBTAG00000013620 and 282110, Uchl5 and IDBG-197081 and ENSMUSG00000018189 and 56207, nuclei, proteasome binding, this GO :0000502 and proteasome complex and cellular component this GO :0004843 and ubiquitin-specific protease activity and molecular function this GO :0004866 and endopeptidase inhibitor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005829 and cytosol and cellular component this GO :0006281 and DNA repair and biological process this GO :0006310 and DNA recombination and biological process this GO :0006351 and transcription, this GO :0004843 : ubiquitin-specific protease activity, this GO :0004843 : ubiquitin-specific protease activity and also this GO :0004866 : endopeptidase inhibitor activity and also this GO :0005515 : protein binding and also this GO :0008242 : omega peptidase activity and also this GO :0044822 : poly(A) RNA binding and also this GO :0070628 : proteasome binding, this GO :0004866 : endopeptidase inhibitor activity, this GO :0005515 : protein binding, this GO :0008242 : omega peptidase activity, this GO :0044822 : poly(A) RNA binding, this GO :0070628 : proteasome binding, ubiquitin carboxyl-terminal hydrolase L5 |
| Identity: |
19678 |
| Gene: |
UCHL5 |
More about : UCHL5 |
| Long gene name: |
ubiquitin C-terminal hydrolase L5 |
| Synonyms gene name: |
ubiquitin carboxyl-terminal hydrolase L5 |
| Synonyms: |
UCH37 CGI-70 INO80R |
| Synonyms name: |
INO80 complex subunit R |
| Locus: |
1q31, 2 |
| Discovery year: |
2002-11-11 |
| Entrez gene record: |
51377 |
| Pubmed identfication: |
10810093 16027725 |
| RefSeq identity: |
NM_015984 |
| Classification: |
Ubiquitin C-terminal hydrolases INO80 complex |
| Havana BLAST/BLAT: |
OTTHUMG00000035561 |