| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
UCHL3 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the UCHL3 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This UCHL3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P15374 |
| Purity: |
Purified |
| Description: |
(1989) purified the predominant isozyme, All 4 were thiol proteases and had high-affinity binding sites for ubiquitin, By screening a human B-cell expression library with the antibodies, Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus, Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1, There are 2 distinct families of DUBs, UCHL3, Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, Wilkinson et al, and raised antibodies against it, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers, the authors isolated cDNAs encoding human UCHL3, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs), Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes |
| Immunogen: |
A portion of amino acids 195-225 from the human protein was used as the immunogen for this UCHL3 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the UCHL3 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
UCHL3 |
| Short name: |
UCHL3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
UCHL3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
12515 |
| Gene: |
UCHL3 |
More about : UCHL3 |
| Long gene name: |
ubiquitin C-terminal hydrolase L3 |
| Synonyms gene name: |
ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) |
| Synonyms name: |
ubiquitin thiolesterase |
| Locus: |
13q22, 2 |
| Discovery year: |
1991-07-15 |
| GenBank acession: |
M30496 |
| Entrez gene record: |
7347 |
| Pubmed identfication: |
2530630 |
| RefSeq identity: |
NM_006002 |
| Classification: |
Ubiquitin C-terminal hydrolases |
| Havana BLAST/BLAT: |
OTTHUMG00000017090 |