| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PARP 1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the PARP 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This PARP 1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P09874 |
| Purity: |
Antigen affinity |
| Description: |
Mediates the poly(ADP-ribosyl)ation of APLF and CHFR, PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150, Required for PARP9 and DTX3L recruitment to DNA damage sites, This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks, UIMC1/RAP80, With EEF1A1 and TXK, [UniProt], and BRCA1 to DNA damage sites, and is thus involved importantly in Th1 cytokine production, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, Involved in the base excision repair (BER) pathway |
| Immunogen: |
A portion of amino acids 590-618 from the human protein was used as the immunogen for this PARP 1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the PARP 1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PARP 1 |
| Short name: |
PARP 1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PARP 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
270 |
| Gene: |
PARP1 |
More about : PARP1 |
| Long gene name: |
poly(ADP-ribose) polymerase 1 |
| Synonyms gene: |
PPOL ADPRT |
| Synonyms gene name: |
ADP-ribosyltransferase (NAD+, member 1 , poly (ADP-ribose) polymerase) poly (ADP-ribose) polymerase family |
| Synonyms: |
PARP |
| Locus: |
1q42, 12 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
BC037545 |
| Entrez gene record: |
142 |
| Pubmed identfication: |
10964595 |
| RefSeq identity: |
NM_001618 |
| Classification: |
Zinc fingers PARP-type Zinc fingers Poly(ADP-ribose) polymerases |
| Havana BLAST/BLAT: |
OTTHUMG00000037556 |