| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Wee1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the Wee1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Wee1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P47810 |
| Purity: |
Antigen affinity |
| Description: |
A correlated decrease in protein level occurs at M/G1 phase, Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated, Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (By similarity), Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase, probably due to its degradation, Wee1 may act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis |
| Immunogen: |
A portion of amino acids 202-230 from the mouse protein was used as the immunogen for this Wee1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Wee1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Wee1 |
| Short name: |
Wee1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Wee1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
12761 |
| Gene: |
WEE1 |
More about : WEE1 |
| Long gene name: |
WEE1 G2 checkpoint kinase |
| Synonyms gene name: |
pombe) , pombe) homolog WEE1 homolog (S, wee1+ (S |
| Synonyms: |
WEE1A |
| Locus: |
11p15, 4 |
| Discovery year: |
1992-08-06 |
| GenBank acession: |
X62048 |
| Entrez gene record: |
7465 |
| Pubmed identfication: |
1840647 |
| RefSeq identity: |
NM_003390 |
| Havana BLAST/BLAT: |
OTTHUMG00000165863 |