| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ATG-5 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IF, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:200, Western blot: 1:500 |
| Notes: |
Titration of the ATG-5 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This ATG-5 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9H1Y0 |
| Purity: |
Purified |
| Description: |
APG5, APG5 may also play an important role in the apoptotic process, Its expression is a relatively late event in the apoptotic process, Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, The conjugate detaches from the membrane immediately before or after autophagosome formation is completed, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation, occurring downstream of caspase activity, possibly within the modified cytoskeleton, required for autophagy, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole), Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells |
| Immunogen: |
This ATG-5 antibody was produced from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length of human ATG5 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the ATG-5 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ATG-5 |
| Short name: |
ATG-5 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
ATG-5 (Antibody to) |
| Alternative technique: |
antibodies |