| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
LIN28B |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, IF, IHC, WB |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the LIN28B antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This LIN28B antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q6ZN17 |
| Purity: |
Antigen affinity |
| Description: |
Binds primary let-7 transcripts (pri-let-7), Does not act on pri-miR21, Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop, Mediates MYC-induced let-7 repression (By similarity), The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation, This activity might not be relevant in vivo, When overexpressed, [UniProt], and sequester them in the nucleolus, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent, away from the microprocessor complex, hence preventing their processing into mature miRNA, including pri-let-7g and pri-let-7a-1, including that of let-7 and possibly of miR107, leading to their terminal uridylation and degradation, miR-143 and miR-200c, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, LIN28B is a suppressor of microRNA (miRNA) biogenesis |
| Immunogen: |
A portion of amino acids 95-128 from the human protein was used as the immunogen for this LIN28B antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the LIN28B antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
LIN28B |
| Short name: |
LIN28B Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
LIN28B (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
32207 |
| Gene: |
LIN28B |
More about : LIN28B |
| Long gene name: |
lin-28 homolog B |
| Synonyms gene name: |
elegans) , lin-28 homolog B (C |
| Synonyms: |
FLJ16517 CSDD2 |
| Locus: |
6q16, 3-q21 |
| Discovery year: |
2005-08-22 |
| GenBank acession: |
AK131411 |
| Entrez gene record: |
389421 |
| RefSeq identity: |
NM_001004317 |
| Havana BLAST/BLAT: |
OTTHUMG00000015290 |