| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
LIN28B |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, WB |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the LIN28B antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This LIN28B antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q6ZN17 |
| Purity: |
Purified |
| Description: |
A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver, Although predominantly localized in the cytoplasm, Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells, Overexpression of LIN28B was noted in most HCC cell lines and clinical samples, Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene, Similar with lin-28 proteins, The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans, Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma |
| Immunogen: |
A portion of amino acids 218-250 from the human protein was used as the immunogen for this LIN28B antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the LIN28B antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
LIN28B |
| Short name: |
LIN28B Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
LIN28B (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
32207 |
| Gene: |
LIN28B |
More about : LIN28B |
| Long gene name: |
lin-28 homolog B |
| Synonyms gene name: |
elegans) , lin-28 homolog B (C |
| Synonyms: |
FLJ16517 CSDD2 |
| Locus: |
6q16, 3-q21 |
| Discovery year: |
2005-08-22 |
| GenBank acession: |
AK131411 |
| Entrez gene record: |
389421 |
| RefSeq identity: |
NM_001004317 |
| Havana BLAST/BLAT: |
OTTHUMG00000015290 |