| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MCM4 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the MCM4 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This MCM4 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P33991 |
| Purity: |
Antigen affinity |
| Description: |
6 and 7 proteins possesses DNA helicase activity, Alternatively spliced transcript variants encoding the same protein have been reported, The MCM complex consisting of this protein and MCM2, The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins, The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex, This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks, and may act as a DNA unwinding enzyme, The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication |
| Immunogen: |
A portion of amino acids 637-665 from the human protein was used as the immunogen for this MCM4 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the MCM4 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MCM4 |
| Short name: |
MCM4 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
MCM4 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6947 |
| Gene: |
MCM4 |
More about : MCM4 |
| Long gene name: |
minichromosome maintenance complex component 4 |
| Synonyms gene: |
CDC21 |
| Synonyms gene name: |
MCM4 minichromosome maintenance deficient 4 (S, cerevisiae) |
| Synonyms: |
CDC54 hCdc21 P1-Cdc21 MGC33310 |
| Locus: |
8q11, 21 |
| Discovery year: |
1994-12-13 |
| Entrez gene record: |
4173 |
| Pubmed identfication: |
7601140 |
| RefSeq identity: |
NM_005914 |
| Classification: |
MCM family |
| Havana BLAST/BLAT: |
OTTHUMG00000164205 |