| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MCM4 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the MCM4 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This MCM4 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P33991 |
| Purity: |
Antigen affinity |
| Description: |
4, CDK phosphorylation at the distal part of the Mcm4 NSD becomes crucial, In the absence of DDK, MCM4 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication, S, The 864-amino acid protein has an observed molecular mass of 97 kD by SDS-PAGE, The gene encodes a subunit of the MCM2-7 complex (also known as MCM2-MCM7), Western blot analysis with and without phosphatase treatment suggested that MCM4 is highly phosphorylated in mitotic cells, also called CDC21, cerevisiae, homolog of, is a protein that in humans is encoded by the MCM4 gene, the replication licensing factor and presumptive replicative helicase, which is similar to its calculated molecular mass of 96, 6 kD, Minichromosome maintenance |
| Immunogen: |
mouse and rat), An amino acid sequence from the C-terminus of human MCM4 (TPALKYQQLFEDIRG) was used as the immunogen for this MCM4 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the MCM4 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MCM4 |
| Short name: |
MCM4 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
MCM4 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6947 |
| Gene: |
MCM4 |
More about : MCM4 |
| Long gene name: |
minichromosome maintenance complex component 4 |
| Synonyms gene: |
CDC21 |
| Synonyms gene name: |
MCM4 minichromosome maintenance deficient 4 (S, cerevisiae) |
| Synonyms: |
CDC54 hCdc21 P1-Cdc21 MGC33310 |
| Locus: |
8q11, 21 |
| Discovery year: |
1994-12-13 |
| Entrez gene record: |
4173 |
| Pubmed identfication: |
7601140 |
| RefSeq identity: |
NM_005914 |
| Classification: |
MCM family |
| Havana BLAST/BLAT: |
OTTHUMG00000164205 |