| Alternative name 1: |
Biotin, Clone: F10-89-4, LCA, Mab anti-Human, T200, flow, CD45 |
| Alternative name 2: |
Biotin, Clone: F10-89-4, LCA, Mab antibody-Human, T200, flow, CD45 |
| Alternative name 3: |
Biotin, Clone: F10-89-4, LCA, Mab antibody-Homo sapiens, T200, flow, CD45 |
| Alternative name 4: |
anti-CD45 LCA T200 Clone: F10-89-4 flow |
| Alternative name 5: |
CD45 LCA T200 Clone: F10-89-4 flow |
| Clonality: |
Monoclonal Antibody |
| Description: |
Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, If used correctly and according the protocol, in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications, This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against |
| Storage and handling: |
Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°, for longer periods - at -20 , C, Generally |
| Advisory: |
For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container, Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom, To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Human proteins, Mainly analyzed are human serum, Modern , To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, 6, Biotin conjugates can be detected by horseradish peroxidase, Homo sapiens sapiens), humans , ssp |
| Conjugation: |
Biotinylated |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| French translation: |
anticorps |
| Gene target: |
F10-89-4, LCA, Monoclonal, T200, CD45 |
| Short name: |
Clone: F10-89-4, LCA, Mouse Monoclonal antibody-, T200, CD45 |
| Technique: |
Monoclonals or monoclonal antibodies, mouses, Mouse |
| Label: |
Biotin |
| Species: |
Humans, Mouses, Human |
| Alternative name: |
LCA, Mouse monoclonal (Antibody to)-H, T200, biotinilated, clonality: F10-89-4, flow, sapiens, CD45 |
| Alternative technique: |
murine |
| Identity: |
9666 |
| Gene: |
PTPRC |
More about : PTPRC |
| Long gene name: |
protein tyrosine phosphatase, receptor type C |
| Synonyms gene: |
CD45 |
| Synonyms: |
LCA T200 GP180 |
| Locus: |
1q31, 1 , 3-q32 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
Y00062 |
| Entrez gene record: |
5788 |
| Pubmed identfication: |
2169617 |
| RefSeq identity: |
NM_002838 |
| Classification: |
Fibronectin type III domain containing CD molecules Protein tyrosine phosphatases, receptor type |
| Havana BLAST/BLAT: |
OTTHUMG00000035702 |
| Locus Specific Databases: |
PTPRCbase: Mutation registry for CD45 deficiency LRG_95 |