| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Neutrophil elastase |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the Neutrophil elastase antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Neutrophil elastase antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P08246 |
| Purity: |
Antigen affinity |
| Description: |
All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation, Humans have six elastase genes which encode the structurally similar proteins, Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN), Salmonella and Yersinia, The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix, The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, This gene is clustered with other serine protease gene family members, This protein degrades the outer membrane protein A (OmpA) of E, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils, at chromosome 19pter, azurocidin 1 and proteinase 3 genes, called azurophil granules, coli as well as the virulence factors of such bacteria as Shigella, Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin |
| Immunogen: |
A portion of amino acids 13-39 from the human protein was used as the immunogen for this Neutrophil elastase antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Neutrophil elastase antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Neutrophil elastase |
| Short name: |
Neutrophil elastase Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Neutrophil elastase (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3309 |
| Gene: |
ELANE |
More about : ELANE |
| Long gene name: |
elastase, neutrophil expressed |
| Synonyms gene: |
ELA2 |
| Synonyms gene name: |
elastase 2, neutrophil |
| Synonyms: |
NE HNE HLE |
| Synonyms name: |
neutrophil elastase leukocyte elastase medullasin |
| Locus: |
19p13, 3 |
| Discovery year: |
1989-09-08 |
| Entrez gene record: |
1991 |
| Pubmed identfication: |
2902087 |
| RefSeq identity: |
NM_001972 |
| Havana BLAST/BLAT: |
OTTHUMG00000181839 |
| Locus Specific Databases: |
ELA2base: Mutation registry for Cyclic and congenital neutropenia LRG_57 |