| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Neutrophil Elastase |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, IHC (FFPE): 1-2ug/ml, Western blot: 0 |
| Added buffer: |
025% sodium azide, 5% BSA and 0, Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2 |
| Intented use: |
This Neutrophil Elastase antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q3UP87 |
| Purity: |
Antigen affinity |
| Description: |
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin, Following activation, Humans have six elastase genes which encode structurally similar proteins, Its key physiologic role is in innate host defense, Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN), Salmonella and Yersinia, The encoded preproprotein is proteolytically processed to generate the active protease, This gene is present in a gene cluster on chromosome 19, This protein also degrades the outer membrane protein A (OmpA) of E, also called Elane, as well as proteins of the extracellular matrix, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses, called azurophil granules, coli as well as the virulence factors of such bacteria as Shigella, is a serine protease of neutrophil and monocyte granules, this protease hydrolyzes proteins within specialized neutrophil lysosomes, Neutrophil elastase |
| Immunogen: |
Amino acids S27-N265 from the mouse protein were used as the immunogen for the Neutrophil Elastase antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Neutrophil Elastase antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
Cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Neutrophil Elastase |
| Short name: |
Neutrophil Elastase Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Neutrophil Elastase (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3309 |
| Gene: |
ELANE |
More about : ELANE |
| Long gene name: |
elastase, neutrophil expressed |
| Synonyms gene: |
ELA2 |
| Synonyms gene name: |
elastase 2, neutrophil |
| Synonyms: |
NE HNE HLE |
| Synonyms name: |
neutrophil elastase leukocyte elastase medullasin |
| Locus: |
19p13, 3 |
| Discovery year: |
1989-09-08 |
| Entrez gene record: |
1991 |
| Pubmed identfication: |
2902087 |
| RefSeq identity: |
NM_001972 |
| Havana BLAST/BLAT: |
OTTHUMG00000181839 |
| Locus Specific Databases: |
ELA2base: Mutation registry for Cyclic and congenital neutropenia LRG_57 |