| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Nucleolin |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IF, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Due to differences in protocols and secondary/substrate sensitivity, it may be necessary to titrate the Nucleolin antibody for optimal performance, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Nucleolin antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P19338 |
| Purity: |
Antigen affinity |
| Description: |
Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb, It is located mainly in dense fibrillar regions of the nucleolus, The intron 11 of the NCL gene encodes a small nucleolar RNA, [Wiki], a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes, termed U20, Nucleolin (NCL) |
| Immunogen: |
The amino acid sequence used as immunogen for this Nucleolin antibody is found between 425-455 of the human protein |
| Storage: |
Avoid repeat freeze-thaws, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, The Nucleolin antibody should be aliquoted and and stored frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Nucleolin |
| Short name: |
Nucleolin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Nucleolin (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7667 |
| Gene: |
NCL |
More about : NCL |
| Long gene name: |
nucleolin |
| Synonyms: |
C23 |
| Locus: |
2q37, 1 |
| Discovery year: |
1991-01-15 |
| Entrez gene record: |
4691 |
| Pubmed identfication: |
2394707 3409881 |
| RefSeq identity: |
NM_005381 |
| Classification: |
RNA binding motif containing DNA helicases |
| Havana BLAST/BLAT: |
OTTHUMG00000153866 |