| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
LIN28 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, WB |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the LIN28 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This LIN28 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9H9Z2 |
| Purity: |
Purified |
| Description: |
ARBP/36B4 ribosomal protein mRNA and its own mRNA, Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, Binds IGF2 mRNA, Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells, Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity), In contrast, Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, LIN28A down-regulation in neural stem cells by miR-125, MYOD1 mRNA, Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7, Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation, a miRNA precursor, allows the processing of pre-let-7, driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis, in stabilizing the mRNAs, indirectly, leading to the terminal uridylation of pre-let-7, leading to their terminal uridylation and subsequent degradation, Acts as a 'translational enhancer' |
| Immunogen: |
This LIN28A antibody was produced from rabbits immunized with a His fusion protein from human LIN28A |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the LIN28 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
LIN28 |
| Short name: |
LIN28 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
LIN28 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
15986 |
| Gene: |
LIN28A |
More about : LIN28A |
| Long gene name: |
lin-28 homolog A |
| Synonyms gene: |
LIN28 |
| Synonyms gene name: |
elegans) , lin-28 homolog (C |
| Synonyms: |
LIN-28 FLJ12457 ZCCHC1 CSDD1 |
| Locus: |
1p36, 11 |
| Discovery year: |
2003-07-21 |
| GenBank acession: |
AF521099 |
| Entrez gene record: |
79727 |
| Pubmed identfication: |
12798299 |
| RefSeq identity: |
NM_024674 |
| Classification: |
Zinc fingers CCHC-type |
| Havana BLAST/BLAT: |
OTTHUMG00000003550 |