| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
JMJD3 (KDM6B) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IF, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the JMJD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This JMJD3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O15054 |
| Purity: |
Purified |
| Description: |
A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function, Contributions of histone demethylase activity to tumor development, Family members include JHDM1 (demethylating histone 3 at lysine 36), Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate, While most covalent histone modifications are reversible, and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9), and hormone-dependent transcriptional activation have been observed, decreases in cell proliferation, only recently has it been established that methyl groups are subject to enzymatic removal from histones, Covalent modification of histones plays critical role in regulating chromatin structure and transcription |
| Immunogen: |
A portion of amino acids 1-30 from the human protein was used as the immunogen for this JMJD3 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the JMJD3 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
JMJD3 (KDM6B) |
| Short name: |
JMJD3 Antibody (KDM6B) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
JMJD3 (Antibody to) (KDM6B) |
| Alternative technique: |
antibodies |
| Identity: |
29012 |
| Gene: |
KDM6B |
More about : KDM6B |
| Long gene name: |
lysine demethylase 6B |
| Synonyms gene: |
JMJD3 |
| Synonyms gene name: |
histone lysine demethylase lysine (K)-specific demethylase 6B , jumonji domain containing 3 jumonji domain containing 3 |
| Synonyms: |
KIAA0346 |
| Locus: |
17p13, 1 |
| Discovery year: |
2004-02-27 |
| GenBank acession: |
AB002344 |
| Entrez gene record: |
23135 |
| Pubmed identfication: |
10662545 9205841 |
| RefSeq identity: |
XM_043272 |
| Classification: |
Lysine demethylases |
| Havana BLAST/BLAT: |
OTTHUMG00000178021 |