| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
NEK2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the NEK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This NEK2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P51955 |
| Purity: |
Purified |
| Description: |
Both human NEK2 and fungal NIMA phosphorylate a similar, Like NIMA, NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers, Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation, These observations demonstrate that NEK2 resembles Aspergillus NIMA, This antibody is specific to isoform 2 of NEK2, and cell cycle-dependent expression, both in its catalytic domain, but not identical, reaching maximal levels in late G2, set of proteins and synthetic peptides, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2 |
| Immunogen: |
A portion of amino acids 350-382 from the human protein was used as the immunogen for this NEK2 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the NEK2 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
NEK2 |
| Short name: |
NEK2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
NEK2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7745 |
| Gene: |
NEK2 |
More about : NEK2 |
| Long gene name: |
NIMA related kinase 2 |
| Synonyms gene name: |
NIMA (never in mitosis gene a)-related kinase 2 |
| Synonyms: |
NLK1 NEK2A RP67 PPP1R111 |
| Synonyms name: |
HsPK 21 protein phosphatase 1, regulatory subunit 111 |
| Locus: |
1q32, 3 |
| Discovery year: |
1998-08-26 |
| GenBank acession: |
U11050 |
| Entrez gene record: |
4751 |
| Pubmed identfication: |
8274451 24043777 |
| RefSeq identity: |
NM_002497 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000037121 |