| Long name: |
NEK2 Primary Polyclonal Antibody |
| Also known as: |
NEK2 PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
NEK2 |
| Specificity: |
This is a highly specific antibody against NEK2 |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
5-55/445 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human NEK2 |
| Gene ID number: |
4751 |
| Swiss Prot: |
P51955 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
CEP25 and NINL, Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome, Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC2 and MAD2L1, Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells, Isoform 2, NECAB3 and NPM1, Phosphorylates: PPP1CC, Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2, Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC8, SGOL1, does not, resulting in their displacement from the centrosomes, which is not present in the nucleolus, Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
HSPK 21, HsPK21, NEK2, NEK2A, Never in mitosis A-related kinase 2, NimA-like protein kinase 1, NimA-related protein kinase 2, PPP1R111, RP67, Serine/threonine-protein kinase Nek2, NLK1 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
NEK2 |
| Short name: |
NEK2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
NEK2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7745 |
| Gene: |
NEK2 |
More about : NEK2 |
| Long gene name: |
NIMA related kinase 2 |
| Synonyms gene name: |
NIMA (never in mitosis gene a)-related kinase 2 |
| Synonyms: |
NLK1 NEK2A RP67 PPP1R111 |
| Synonyms name: |
HsPK 21 protein phosphatase 1, regulatory subunit 111 |
| Locus: |
1q32, 3 |
| Discovery year: |
1998-08-26 |
| GenBank acession: |
U11050 |
| Entrez gene record: |
4751 |
| Pubmed identfication: |
8274451 24043777 |
| RefSeq identity: |
NM_002497 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000037121 |