| Category: |
Antibody |
| Concentration: |
09% sodium azide, 4 with 0, In 1X PBS pH 7 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
SIGLEC7 (D-siglec) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the SIGLEC7 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This SIGLEC7 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9Y286 |
| Purity: |
Antigen affinity |
| Description: |
A large subgroup of SIGLECs share high homology with SIGLEC3 (CD33) and are localized to 19q13, Both variants contain 2 C2-set N-terminal Ig-like domains, Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, RT-PCR analysis detected high expression of both variants in spleen and small intestine, SLG-long (SLGL) and SLG-short (SLGS), The 477-amino acid SLGS protein has a weak signal sequence and, The 595-amino acid SLGL protein contains a signal peptide and 2 V-set N-terminal Ig-like domains, The cDNA for the SLG gene encodes 2 variants, The conserved arginine residue thought to be essential for sialic acid binding in other SIGLECs is replaced by a glutamine in SLGS and by a cysteine in SLGL, a transmembrane domain, and SLGS was highly expressed in adrenal gland and SLGL was highly expressed in bone marrow, and a cytoplasmic tail with a putative ITIM and a putative SLAM-like tyrosine-based motif, has only 1 V-set N-terminal Ig-like domain, like most SIGLEC3-like SIGLECs, or ITIMs, 4, SIGLECs are cell surface proteins of the Ig superfamily |
| Immunogen: |
A portion of amino acids 1-30 from the human protein was used as the immunogen for this SIGLEC7 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the SIGLEC7 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
SIGLEC7 (D-siglec) |
| Short name: |
SIGLEC7 Antibody (D-siglec) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
sialic acid binding Ig-like lectin 7 (Antibody to) (D-siglec) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
Plasma membranes, SIGLEC7 and IDBG-65680 and ENSG00000168995 and 27036, carbohydrate binding, this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005887 and integral component of plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0030246 and carbohydrate binding and molecular function this GO :0045087 and innate immune response and biological process, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0005515 : protein binding and also this GO :0030246 : carbohydrate binding, this GO :0005515 : protein binding, this GO :0030246 : carbohydrate binding, sialic acid binding Ig-like lectin 7 |
| Identity: |
10876 |
| Gene: |
SIGLEC7 |
More about : SIGLEC7 |
| Long gene name: |
sialic acid binding Ig like lectin 7 |
| Synonyms gene: |
SIGLEC19P SIGLECP2 |
| Synonyms gene name: |
pseudogene 2 sialic acid binding Ig-like lectin 7 , pseudogene sialic acid binding Ig-like lectin, sialic acid binding Ig-like lectin 19 |
| Synonyms: |
SIGLEC-7 p75/AIRM1 QA79 CD328 |
| Locus: |
19q13, 41 |
| Discovery year: |
2000-01-26 |
| GenBank acession: |
AF170485 |
| Entrez gene record: |
27036 |
| Pubmed identfication: |
10567377 |
| RefSeq identity: |
NM_016543 |
| Classification: |
CD molecules Sialic acid binding Ig like lectins V-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000182895 |