| Antigen detectable with this ELISA kit: |
Synaptosomal Associated Protein 25kDa (SNAP25) |
| Species reactivity: |
Bovine (Cattle), Equine (Horse), Porcine (Pig), Bos taurus, Chicken (Gallus), Equus caballus, Homo sapiens (Human), Mus musculus (Mouse), Oryctolagus cuniculus (Rabbit), Rattus norvegicus (Rat), Sus scrofa |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
055ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synaptosomal Associated Protein 25kDa (SNAP25), The concentration of Synaptosomal Associated Protein 25kDa (SNAP25) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synaptosomal Associated Protein 25kDa (SNAP25), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Synaptosomal Associated Protein 25kDa (SNAP25), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Synaptosomal Associated Protein 25kDa (SNAP25) |
| Short name: |
Synaptosomal Associated Protein 25kDa (SNAP25) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
25kDa) Enzyme-linked immunosorbent assay reagent, Synaptosomal Associated Protein 25kDa (synaptosomal-associated protein |
| Alternative technique: |
elisas |
| Alternative to gene target: |
25kDa, Cell surfaces, SNAP25 and IDBG-53941 and ENSG00000132639 and 6616, SNAP25 and IDBG-636453 and ENSBTAG00000008323 and 540853, Snap25 and IDBG-207842 and ENSMUSG00000027273 and 20614, bA416N4, protein binding, this GO :0000149 and SNARE binding and molecular function this GO :0001504 and neurotransmitter uptake and biological process this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005802 and trans- this GO lgi network and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006112 and energy reserve metabolic process and biological process this GO :0007268 and synaptic transmission and biological process this GO :0007269 and neurotransmitter secretion and biological process this GO :0010975 and regulation of neuron projection development and biological process this GO :0014047 and glutamate secretion and biological process this GO :0016020 and membrane and cellular component this GO :0016081 and synaptic vesicle docking involved in exocytosis and biological process this GO :0016192 and vesicle-mediated transport and biological process this GO :0030054 and cell junction and cellular component this GO :0030426 and growth cone and cellular component this GO :0031083 and BLOC-1 complex and cellular component this GO :0031201 and SNARE complex and cellular component this GO :0043005 and neuron projection and cellular component this GO :0044281 and small molecule metabolic process and biological process this GO :0045202 and synapse and cellular component this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050796 and regulation of insulin secretion and biological process this GO :0060291 and long-term synaptic potentiation and biological process this GO :0070032 and synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex and cellular component this GO :0070201 and regulation of establishment of protein localization and biological process, this GO :0000149 : SNARE binding, this GO :0000149 : SNARE binding and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, 2 and RIC-4 and RIC4 and SEC9 and SNAP and SNAP-25, 2 and dJ1068F16, synaptosomal-associated protein |
| Identity: |
11132 |
| Gene: |
SNAP25 |
More about : SNAP25 |
| Long gene name: |
synaptosome associated protein 25 |
| Synonyms gene: |
SNAP |
| Synonyms gene name: |
25kDa , synaptosomal-associated protein |
| Synonyms: |
SNAP-25 RIC-4 RIC4 SEC9 bA416N4, 2 , 2 dJ1068F16 |
| Synonyms name: |
resistance to inhibitors of cholinesterase 4 homolog |
| Locus: |
20p12, 2 |
| Discovery year: |
1995-01-24 |
| Entrez gene record: |
6616 |
| Pubmed identfication: |
8661740 10692432 |
| RefSeq identity: |
NM_130811 |
| Classification: |
SNAREs |
| Havana BLAST/BLAT: |
OTTHUMG00000031863 |