| Antigen detectable with this ELISA kit: |
Salt Inducible Kinase 2 (SIK2) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
058ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Salt Inducible Kinase 2 (SIK2), The concentration of Salt Inducible Kinase 2 (SIK2) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Salt Inducible Kinase 2 (SIK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Salt Inducible Kinase 2 (SIK2), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Salt Kinase 2 (SIK2) |
| Short name: |
Salt Kinase 2 (SIK2) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Salt Inducible phosphorylation catalyst 2 (salt-inducible kinase 2) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
LOH11CR1I and QIK and SNF1LK2, SIK2 and IDBG-630203 and ENSBTAG00000020272 and 539570, SIK2 and IDBG-70866 and ENSG00000170145 and 23235, Sik2 and IDBG-164487 and ENSMUSG00000037112 and 235344, nuclei, this GO :0000287 and magnesium ion binding and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0016772 and transferase activity, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups, transferring phosphorus-containing groups and molecular function this GO :0035556 and intracellular signal transduction and biological process this GO :0046626 and regulation of insulin receptor signaling pathway and biological process this GO :0046777 and protein autophosphorylation and biological process, salt-inducible kinase 2 |
| Identity: |
21680 |
| Gene: |
SIK2 |
More about : SIK2 |
| Long gene name: |
salt inducible kinase 2 |
| Synonyms gene: |
SNF1LK2 |
| Synonyms gene name: |
SNF1-like kinase 2 |
| Synonyms: |
KIAA0781 QIK DKFZp434K1115 LOH11CR1I |
| Locus: |
11q23, 1 |
| Discovery year: |
2005-01-21 |
| GenBank acession: |
AB018324 |
| Entrez gene record: |
23235 |
| Pubmed identfication: |
15067358 |
| RefSeq identity: |
NM_015191 |
| Havana BLAST/BLAT: |
OTTHUMG00000150644 |