| Antigen detectable with this ELISA kit: |
High Sensitive Interleukin 8 (IL8) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1, 1, 3, 6, 12, 25pg/mL, 50pg/mL, 125pg/mL, 25pg/mL, 563-100pg/mL The standard curve concentrations used for the ELISA&rsquo, 563pg/mL, 5pg/mL, s were 100pg/mL |
| Sensitivity: |
51pg/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 8 (IL8), The concentration of High Sensitive Interleukin 8 (IL8) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 8 (IL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain High Sensitive Interleukin 8 (IL8), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene: |
CXCL8 |
More about : CXCL8 |
| Gene target: |
Sensitive Interleukin 8 (IL8) |
| Short name: |
High Sensitive Interleukin 8 (IL8) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
High Sensitive Interleukin 8 (interleukin 8) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Extracellular, GCP-1 and GCP1 and IL8 and LECT and LUCT and LYNAP and MDNCF and MONAP and NAF and NAP-1 and NAP1, IL8 and IDBG-23954 and ENSG00000169429 and 3576, IL8 and IDBG-643010 and ENSBTAG00000019716 and 280828, chemokine activity, this GO :0001525 and angiogenesis and biological process this GO :0002237 and response to molecule of bacterial origin and biological process this GO :0005153 and interleukin-8 receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006928 and cellular component movement and biological process this GO :0006935 and chemotaxis and biological process this GO :0006954 and inflammatory response and biological process this GO :0006955 and immune response and biological process this GO :0006987 and activation of signaling protein activity involved in unfolded protein response and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007165 and signal transduction and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0008009 and chemokine activity and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0019722 and calcium-mediated signaling and biological process this GO :0030155 and regulation of cell adhesion and biological process this GO :0030593 and neutrophil chemotaxis and biological process this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0031623 and receptor internalization and biological process this GO :0034976 and response to endoplasmic reticulum stress and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0042119 and neutrophil activation and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0044344 and cellular response to fibroblast growth factor stimulus and biological process this GO :0045087 and innate immune response and biological process this GO :0045091 and regulation of retroviral genome replication and biological process this GO :0045744 and negative regulation of G-protein coupled receptor protein signaling pathway and biological process this GO :0048566 and embryonic digestive tract development and biological process this GO :0050930 and induction of positive chemotaxis and biological process this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0071347 and cellular response to interleukin-1 and biological process this GO :0071356 and cellular response to tumor necrosis factor and biological process this GO :0090023 and positive regulation of neutrophil chemotaxis and biological process, this GO :0005153 : interleukin-8 receptor binding, this GO :0005153 : interleukin-8 receptor binding and also this GO :0005515 : protein binding and also this GO :0008009 : chemokine activity, this GO :0005515 : protein binding, this GO :0008009 : chemokine activity, interleukin 8 |
| Identity: |
6025 |
| Long gene name: |
C-X-C motif chemokine ligand 8 |
| Synonyms gene: |
IL8 |
| Synonyms gene name: |
interleukin 8 chemokine (C-X-C motif) ligand 8 |
| Synonyms: |
SCYB8 LUCT LECT MDNCF TSG-1 IL-8 NAP-1 3-10C MONAP AMCF-I LYNAP NAF b-ENAP GCP-1 K60 GCP1 NAP1 |
| Synonyms name: |
neutrophil-activating peptide 1 granulocyte chemotactic protein 1 monocyte-derived neutrophil chemotactic factor lung giant cell carcinoma-derived chemotactic protein tumor necrosis factor-induced gene 1 monocyte-derived neutrophil-activating peptide lymphocyte derived neutrophil activating peptide beta endothelial cell-derived neutrophil activating peptide alveolar macrophage chemotactic factor I |
| Locus: |
4q13, 3 |
| Discovery year: |
1989-06-30 |
| GenBank acession: |
Y00787 |
| Entrez gene record: |
3576 |
| Pubmed identfication: |
1427896 11178128 |
| RefSeq identity: |
NM_000584 |
| Classification: |
Endogenous ligands Chemokine ligands Interleukins |
| Havana BLAST/BLAT: |
OTTHUMG00000151316 |