| Gene info: |
Although TPH catalyzes the same reaction within the raphe nuclei and the pineal gland, Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH, In mammals, Serotonin functions mainly as a neurotransmitter, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis, The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II), and melatonin synthesis takes place within the pineal gland, serotonin biosynthesis occurs predominantly in neurons which originate in the raphe nuclei of the brain, whereas melatonin is the principal hormone secreted by the pineal gland, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin), Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan |
| Gene names: |
Tph, Tryptophan 5-monooxygenase 1, Tph |
| Antibodie's label: |
unlabelled serum |
| Purity: |
Affinity Purified |
| Ab raised in: |
Rabbit |
| Type: |
Polyclonal |
| Spicific to: |
Tryptophan Hydroxylase antibody is specific immunolabeling of the ~55k TPH protein phosphorylated at Ser58 in Western blots of human dorsal Raphe nucleus |
| Antigen: |
TPH (Ser58) polyclonal antibody was made against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphoSer58 of TPH |
| Immuno application: |
Western Blot |
| Antibody advice: |
When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB, Applications include Dot Blots (DB) and Western Blots (WB) |
| Suspention, pH, azide: |
100 ug per mL BSA and 50% glycerol, 150 mM NaCl, 100 uL in 10 mM HEPES (pH 7, 5) |
| Keep at: |
Stable for one year, but shorter term storage at &ndash, 20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol, 80 grades C is recommended, For long term storage &ndash |
| Works with: |
Mouse, Rat, Human |
| Omim nr: |
136122 |
| Ncbi nr: |
P09810 |
| KDa: |
55 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
24848 |
| GENE symbol: |
Tph1 |
| Antigene name: |
tryptophan hydroxylase 1 |
| Source: |
Rattus norvegicus |
| Protein nr: |
P09810 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
Tryptophan (phospho Ser58) Hydroxylase |
| Short name: |
anti-Tryptophan (phospho Ser58) Hydroxylase Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-Tryptophan (phosphorilated Ser58) Hydroxylase (Antibody to) |
| Alternative technique: |
antibodies |