| Gene info: |
In most cell contexts, Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor, The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (A and B forms), These phosphorylation events may differentially affect the transcriptional activity of the receptor, Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones, There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer |
| Gene names: |
NR3C3, Nuclear receptor subfamily 3 group C member 3, PR, PR |
| Antibodie's label: |
unlabelled serum |
| Purity: |
Protein G purified |
| Ab raised in: |
Mouse |
| Type: |
Monoclonal |
| Monoclonal: |
608 |
| Immunoglobulin: |
IgG1 |
| Spicific to: |
Progesterone Receptor antibody is specific for the human progesterone receptor phosphorylated at Ser294 |
| Antigen: |
Progesterone receptor (Ser294) monoclonal antibody was made against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer294of human progesterone receptor |
| Immuno application: |
immuno-histo-chemistry, Western Blot |
| Antibody advice: |
(2000), Applications include Immunoblotting (IB), IHC and WB, Immunohistochemistry (IHC) and Western Blots (WB), Initial recommended range of dilutions: 1:1000 to 1:2000, When internally tested under ideal conditions the working dilutions were 1:1000 for IB, cited above, et al, the antibody specifically reacts with the progesterone receptor phosphorylated at Ser294 in T47D cells, As shown in Clemm |
| Suspention, pH, azide: |
100 ug per mL BSA and 50% glycerol, 150 mM NaCl, 100 uL in 10 mM HEPES (pH 7, 5) |
| Keep at: |
Stable for one year, but shorter term storage at &ndash, 20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol, 80 grades C is recommended, For long term storage &ndash |
| Works with: |
Human |
| Omim nr: |
90110048 |
| Ncbi nr: |
P06401 |
| KDa: |
90/120 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
5241 |
| GENE symbol: |
PGR |
| Antigene name: |
progesterone receptor |
| Source: |
Homo sapiens |
| Protein nr: |
P06401 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
Progesterone Receptor (phospho Ser294) [608] |
| Short name: |
anti-Progesterone Receptor (phospho Ser294) Antibody [608] |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-Progesterone Receptor (phosphorilated Ser294) (Antibody to) [608] |
| Alternative technique: |
antibodies |