| Gene info: |
Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK, Activation of MEK 1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222), This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade, MEK 1 and MEK 2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain |
| Gene names: |
ERK activator kinase 1, MAP kinase kinase 1, MAPKK1, MEK1, MKK1, PRKMK1, CFC3 |
| Antibodie's label: |
unlabelled serum |
| Purity: |
Affinity Purified |
| Ab raised in: |
Rabbit |
| Type: |
Polyclonal |
| Spicific to: |
MEK1/2 antibody is specific for MEK 1/2 phosphorylated at Ser218/222 |
| Antigen: |
MEK 1/2 (Ser218/222) polyclonal antibody was made against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer218/222 of human MEK 1/2 |
| Immuno application: |
Western Blot |
| Antibody advice: |
Applications include Dot Blots (DB) and Western Blots (WB), Human, Suitability for Immunohistochemistry (IHC) has not yet been determined, The immunolabeling is blocked by the phosphopeptide used as antigen but not the corresponding non-phosphopeptide, When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody, The antibody immunolabels the ~45k MEK protein in a Western blot of serum-stimulated NIH 3T3 cells |
| Suspention, pH, azide: |
100 ug per mL BSA and 50% glycerol, 150 mM NaCl, 100 uL in 10 mM HEPES (pH 7, 5) |
| Keep at: |
Stable for one year, but shorter term storage at &ndash, 20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol, 80 grades C is recommended, For long term storage &ndash |
| Works with: |
Mouse, Rat, Xenopus, Human |
| Omim nr: |
400274 |
| Ncbi nr: |
Q02750 |
| KDa: |
45 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
5604 |
| GENE symbol: |
MAP2K1 |
| Antigene name: |
mitogen-activated protein kinase kinase 1 |
| Source: |
Homo sapiens |
| Protein nr: |
Q02750 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
MEK1 / 2 (phospho Ser218 / Ser222) |
| Short name: |
anti-MEK1 / 2 (phospho Ser218 / Ser222) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-MEK1 / 2 (phosphorilated Ser218 / Ser222) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6840 |
| Gene: |
MAP2K1 |
More about : MAP2K1 |
| Long gene name: |
mitogen-activated protein kinase kinase 1 |
| Synonyms gene: |
PRKMK1 |
| Synonyms: |
MEK1 MAPKK1 |
| Locus: |
15q22, 31 |
| Discovery year: |
1993-11-05 |
| GenBank acession: |
L11284 |
| Entrez gene record: |
5604 |
| Pubmed identfication: |
9465908 8388392 |
| Classification: |
Mitogen-activated protein kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000133196 |
| Locus Specific Databases: |
The Globin Gene Server LRG_725 |