| Gene info: |
MARCKS is a member of a family of calmodulin binding proteins, Moreover, Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin, The phosphorylation of Ser152/156 can be used as a measure of PKC activation, Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) |
| Gene names: |
MARCKS, Macs, Protein kinase C substrate 80 kDa protein, KINC |
| Antibodie's label: |
unlabelled serum |
| Purity: |
Affinity Purified |
| Ab raised in: |
Rabbit |
| Type: |
Polyclonal |
| Spicific to: |
MARCKS antibody is specific for MARCKS phosphorylated at Ser152 and Ser156 |
| Antigen: |
MARCKS (Ser152/156) polyclonal antibody was made against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser152 and phospho-Ser156 of rat MARCKS |
| Immuno application: |
Western Blot |
| Antibody advice: |
Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state, Applications include Dot Blots (DB) and Western Blot (WB), Human, Initial recommended range of dilutions: 1:500 to 1:2000, Suitability for Immunohistochemistry (IHC) not yet determined, The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns, When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB, and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody, mouse, rat, Immunolabeling of the 87kDa band in rat brain homogenate is blocked by the Ser152/156 phosphopeptide but not by the non-phosphopeptide |
| Suspention, pH, azide: |
100 ug per mL BSA and 50% glycerol, 150 mM NaCl, 100 uL in 10 mM HEPES (pH 7, 5) |
| Keep at: |
Stable for one year, but shorter term storage at &ndash, 20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol, 80 grades C is recommended, For long term storage &ndash |
| Works with: |
Mouse, Rat, Xenopus, Human |
| Omim nr: |
266495 |
| Ncbi nr: |
P30009 |
| KDa: |
87 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
25603 |
| GENE symbol: |
Marcks |
| Antigene name: |
myristoylated alanine rich protein kinase C substrate |
| Source: |
Rattus norvegicus |
| Protein nr: |
P30009 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
MARCKS (phospho Ser152 / 156) |
| Short name: |
anti-MARCKS (phospho Ser152 / 156) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-MARCKS (phosphorilated Ser152 / 156) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6759 |
| Gene: |
MARCKS |
More about : MARCKS |
| Long gene name: |
myristoylated alanine rich protein kinase C substrate |
| Synonyms gene: |
MACS |
| Synonyms gene name: |
80K-L) , myristoylated alanine-rich protein kinase C substrate (MARCKS |
| Synonyms: |
PKCSL 80K-L |
| Locus: |
6q21 |
| Discovery year: |
1990-01-05 |
| GenBank acession: |
M68956 |
| Entrez gene record: |
4082 |
| Pubmed identfication: |
1560845 8420923 |
| RefSeq identity: |
NM_002356 |
| Havana BLAST/BLAT: |
OTTHUMG00000188327 |