| Gene info: |
The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2, Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle |
| Gene names: |
CDC-like kinase 2, Protein Clk2, LOC365842 |
| Antibodie's label: |
unlabelled serum |
| Purity: |
Affinity Purified |
| Ab raised in: |
Rabbit |
| Type: |
Polyclonal |
| Spicific to: |
Cdc2 antibody is specific for cdc2 phosphorylated at Tyr15 |
| Antigen: |
mouse, rat and xenopus cdc2, Cdc2 (Tyr15) polyclonal antibody was made against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Tyr15 of human |
| Immuno application: |
Western Blot |
| Antibody advice: |
Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state, Applications include Dot Blots (DB) and Western Blot (WB), Drosophila, Immunolabeling of the Mr 34 kDa cdc2 protein is blocked by the Ser15 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide, Initial recommended range of dilutions: 1:500 to 1:2000, Suitability for Immunohistochemistry (IHC) has not been determined, Western blots of SK-N-MC total cell extracts with phospho- and non-phosphopeptide competition were used to establish the phospho-specificity of the antibody, When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB, human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody, The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns |
| Suspention, pH, azide: |
100 ug per mL BSA and 50% glycerol, 150 mM NaCl, 100 uL in 10 mM HEPES (pH 7, 5) |
| Keep at: |
Stable for one year, but shorter term storage at &ndash, 20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol, 80 grades C is recommended, For long term storage &ndash |
| Works with: |
Dog, Mouse, Rat, Xenopus, Human |
| Omim nr: |
81910362 |
| Ncbi nr: |
Q5XI98 |
| KDa: |
34 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
365842 |
| GENE symbol: |
Clk2 |
| Antigene name: |
CDC-like kinase 2 |
| Source: |
Rattus norvegicus |
| Protein nr: |
Q5XI98 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
Cdc2 (phospho Tyr15) |
| Short name: |
anti-Cdc2 (phospho Tyr15) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-Cdc2 (phosphorilated Tyr15) (Antibody to) |
| Alternative technique: |
antibodies |