| Gene info: |
Autophosphorylation of Thr305 inhibits the activity CaM Kinase II, Phosphorylation at this site appears to reduce the association of CaM kinase II with the PSD and reduce LTP and learning, The antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr305 found in rat brain CaM Kinase II, Calcium/calmodulin kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to second messengers |
| Gene names: |
CaM kinase II subunit alpha, PK2CDD, PKCCD |
| Antibodie's label: |
unlabelled serum |
| Purity: |
Neat Serum |
| Ab raised in: |
Rabbit |
| Type: |
Polyclonal |
| Spicific to: |
CaM Kinase II antibody is specific for a 50k and b 60k CaM Kinase II phosphorylated at Thr305 |
| Antigen: |
CaM Kinase II (Thr305) polyclonal antibody was made against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr305 found in rat brain CaM Kinase II |
| Immuno application: |
Western Blot |
| Antibody advice: |
Applications include Dot Blot (DB) and Western Blot (WB), Bovine, Dot Blots and Western Blots performed with each lot, Immunolabeling is absent in homogenate of brain from T305D mutant mice, Suitability for Immunohistochemistry (IHC) has not been determined, The labeling of the CaM Kinase II bands in a Western blot of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide, Western blots of rat brain lysates with phospho- and dephosphopeptide competition were used to establish the phospho-specificity of the antibody, and internal testing under ideal conditions concluded a working dilution of 1:1000, chicken, human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody, Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state |
| Keep at: |
Stable for one year, but shorter term storage at &ndash, 20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol, 80 grades C is recommended, For long term storage &ndash |
| Works with: |
Bovine, Chicken, Mouse, Rat, Xenopus, Human |
| Omim nr: |
125285 |
| Ncbi nr: |
P11275 |
| KDa: |
50/60 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
25400 |
| GENE symbol: |
Camk2a |
| Antigene name: |
calcium/calmodulin-dependent protein kinase II alpha |
| Source: |
Rattus norvegicus |
| Protein nr: |
P11275 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
CaM Kinase II (phospho Thr305) |
| Short name: |
anti-CaM Kinase II (phospho Thr305) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-CaM phosphorylation catalyst II (phosphorilated Thr305) (Antibody to) |
| Alternative technique: |
antibodies |