| Antigen detectable with this ELISA kit: |
Member 4 (WNT4), Wingless Type MMTV Integration Site Family |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
054ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Member 4 (WNT4), Member 4 (WNT4), Member 4 (WNT4), Member 4 (WNT4) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, The concentration of Wingless Type MMTV Integration Site Family, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Wingless Type MMTV Integration Site Family, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Member 4 (WNT4), Wingless MMTV Integration Site Family |
| Short name: |
Member 4 (WNT4) ELISA kit, Wingless MMTV Integration Site Family |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Member 4 (wingless-classification MMTV integration site family, member 4) Enzyme-linked immunosorbent assay reagent, Wingless classification MMTV Integration Site family |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0048018 and receptor a this GO nist activity and molecular function this GO :0048599 and oocyte development and biological process this GO :0048754 and branching morphogenesis of an epithelial tube and biological process this GO :0048856 and anatomical structure development and biological process this GO :0051145 and smooth muscle cell differentiation and biological process this GO :0051496 and positive regulation of stress fiber assembly and biological process this GO :0051894 and positive regulation of focal adhesion assembly and biological process this GO :0060070 and canonical Wnt signaling pathway and biological process this GO :0060126 and somatotropin secreting cell differentiation and biological process this GO :0060129 and thyroid-stimulating hormone-secreting cell differentiation and biological process this GO :0060231 and mesenchymal to epithelial transition and biological process this GO :0060748 and tertiary branching involved in mammary gland duct morphogenesis and biological process this GO :0060993 and kidney morphogenesis and biological process this GO :0061045 and negative regulation of wound healing and biological process this GO :0061180 and mammary gland epithelium development and biological process this GO :0061184 and positive regulation of dermatome development and biological process this GO :0061205 and paramesonephric duct development and biological process this GO :0061369 and negative regulation of testicular blood vessel morphogenesis and biological process this GO :0071560 and cellular response to transforming growth factor beta stimulus and biological process this GO :0072033 and renal vesicle formation and biological process this GO :0072034 and renal vesicle induction and biological process this GO :0072162 and metanephric mesenchymal cell differentiation and biological process this GO :0072164 and mesonephric tubule development and biological process this GO :0072174 and metanephric tubule formation and biological process this GO :0072210 and metanephric nephron development and biological process this GO :0072273 and metanephric nephron morphogenesis and biological process this GO :0090002 and establishment of protein localization to plasma membrane and biological process this GO :0090090 and negative regulation of canonical Wnt signaling pathway and biological process this GO :0090263 and positive regulation of canonical Wnt signaling pathway and biological process this GO :2000019 and negative regulation of male this GO nad development and biological process this GO :2000066 and positive regulation of cortisol biosynthetic process and biological process this GO :2000225 and negative regulation of testosterone biosynthetic process and biological process this GO :2001234 and negative regulation of apoptotic signaling pathway and biological process, Extracellular, WNT4 and IDBG-645527 and ENSBTAG00000022671 and 789600, WNT4 and IDBG-93664 and ENSG00000162552 and 54361, Wnt4 and IDBG-198435 and ENSMUSG00000036856 and 22417, member 4, receptor a this GO nist activity, this GO :0001656 and metanephros development and biological process this GO :0001658 and branching involved in ureteric bud morphogenesis and biological process this GO :0001822 and kidney development and biological process this GO :0001823 and mesonephros development and biological process this GO :0001837 and epithelial to mesenchymal transition and biological process this GO :0001838 and embryonic epithelial tube formation and biological process this GO :0001889 and liver development and biological process this GO :0003714 and transcription corepressor activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005109 and frizzled binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005578 and proteinaceous extracellular matrix and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0005796 and this GO lgi lumen and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006702 and androgen biosynthetic process and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007276 and gamete generation and biological process this GO :0007548 and sex differentiation and biological process this GO :0008584 and male this GO nad development and biological process this GO :0008585 and female this GO nad development and biological process this GO :0009267 and cellular response to starvation and biological process this GO :0009986 and cell surface and cellular component this GO :0010629 and negative regulation of gene expression and biological process this GO :0010894 and negative regulation of steroid biosynthetic process and biological process this GO :0016055 and Wnt signaling pathway and biological process this GO :0018345 and protein palmitoylation and biological process this GO :0022407 and regulation of cell-cell adhesion and biological process this GO :0030154 and cell differentiation and biological process this GO :0030182 and neuron differentiation and biological process this GO :0030237 and female sex determination and biological process this GO :0030325 and adrenal gland development and biological process this GO :0030336 and negative regulation of cell migration and biological process this GO :0030501 and positive regulation of bone mineralization and biological process this GO :0031012 and extracellular matrix and cellular component this GO :0032321 and positive regulation of Rho GTPase activity and biological process this GO :0032349 and positive regulation of aldosterone biosynthetic process and biological process this GO :0032967 and positive regulation of collagen biosynthetic process and biological process this GO :0033077 and T cell differentiation in thymus and biological process this GO :0033080 and immature T cell proliferation in thymus and biological process this GO :0035239 and tube morphogenesis and biological process this GO :0035567 and non-canonical Wnt signaling pathway and biological process this GO :0038030 and non-canonical Wnt receptor signaling pathway via MAPK cascade and biological process this GO :0040037 and negative regulation of fibroblast growth factor receptor signaling pathway and biological process this GO :0042445 and hormone metabolic process and biological process this GO :0045165 and cell fate commitment and biological process this GO :0045596 and negative regulation of cell differentiation and biological process this GO :0045669 and positive regulation of osteoblast differentiation and biological process this GO :0045836 and positive regulation of meiosis and biological process this GO :0045892 and negative regulation of transcription, this GO :0003714 : transcription corepressor activity, this GO :0003714 : transcription corepressor activity and also this GO :0005102 : receptor binding and also this GO :0005109 : frizzled binding and also this GO :0005515 : protein binding and also this GO :0048018 : receptor a this GO nist activity, this GO :0005102 : receptor binding, this GO :0005109 : frizzled binding, this GO :0005515 : protein binding, this GO :0048018 : receptor a this GO nist activity, wingless-type MMTV integration site family |
| Identity: |
12783 |
| Gene: |
WNT4 |
More about : WNT4 |
| Long gene name: |
Wnt family member 4 |
| Synonyms gene name: |
member 4 , wingless-type MMTV integration site family |
| Synonyms: |
WNT-4 |
| Locus: |
1p36, 12 |
| Discovery year: |
2000-07-31 |
| GenBank acession: |
AL031281 |
| Entrez gene record: |
54361 |
| Pubmed identfication: |
8168088 |
| Classification: |
Wnt family Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000002894 |