| Lysates properties: |
EDTA, For quality control purposes, Glycerol, KCl, MgCl2, NP-40, Sucrose, The human diaphragm tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 grades C, The isolated diaphragm tissue membrane protein is then Western analyzed by either GAPDH or &, The membrane protein is provided in a buffer including HEPES (pH 7, and a cocktail of protease inhibitors, sodium deoxycholate, the isolated diaphragm tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining, #946, -actin antibody to confirm there is no signal or very weak signal, 9), Human diaphragm tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique |
| Lysate's origin: |
Tissue |
| State of tissue: |
Normal |
| Species of the lysate: |
Human |
| Cellular origibn of the lysate: |
Diaphragm |
| Disease of the lysate: |
Normal |
| Localisation of the biopsy: |
Human Diaphragm tissue |
| Ug of lysate used by test: |
10 ug to 20 ug per lane is recommended for mini gel, Diaphragm membrane protein lysate is for use in Western blotting |
| Positive control in: |
the lysate is used as positive control in Western Blot |
| PH and medium: |
EDTA, Glycerol, KCl, MgCl2, Sodium deoxycholate, Sucrose, and NP-40, 9, HEPES pH 7 |
| Keep at: |
For extended storage, Repeated freezing and thawing is not recommended, Under proper storage conditions the shelf life is half a year from the date of receipt, freeze working aliquots at -70 grades C, Store at 2-8 grades C for continuous use |
| Caution: |
This product is for research use only |
| Laboratory requirements: |
Also, Centers for Disease Control and Prevention's Office of Health and Safety (www, Department of Health and Human Services, GENTAUR is not liable for damages or injuries resulting from receipt and/or use of GENTAUR materials, Please refer to the Material Safety Data Sheet (MSDS) for safe storage, They are not intended for use in humans, and use procedures, for further information on the biosafety classification of human etiologic agents, handling, please consult the U, Products are intended for laboratory research purposes only and should be used by qualified personnel only, S, cdc, gov/od/ohs) |
| Animal origin: |
Human |
| Dilution: |
2 mg/mL or more concentrated |
| Gene target: |
Diaphragm Membrane Lysate |
| Short name: |
Diaphragm Membrane Lysate |
| Alternative name: |
Diaphragm Membrane Lysate |