| Gene names: |
A proliferation-inducing ligand, APRIL, CD256, TALL-2, TALL2, TRDL-1, Tumor necrosis factor ligand superfamily member 13, UNQ383/PRO715, ZTNF2, APRIL |
| Antibodie's label: |
unlabelled serum |
| Purity: |
=95% in SDS-PAGE, APRIL (monoclonal Aprily-8) antibody was purified to > |
| Ab raised in: |
Mouse |
| Type: |
Monoclonal |
| Monoclonal: |
Aprily-8 |
| Immunoglobulin: |
IgG1 |
| Antigen: |
93-233) expressed in bacteria, APRIL (monoclonal Aprily-8) antibody was made against recombinant human APRIL (aa |
| Immuno application: |
FLOW, immuno-cyto-chemistry, Western Blot |
| Antibody advice: |
0, 100% EtOH and xylol, 2x 10min in 100% EtOH, 5% fetal calf serum, 60% EtOH and PBS, 80%, 80%, 90%, After PBS washes, After a wash in PBS, After micro-wave treatment, After washing in FACS buffer, Cells (5x105) were incubated on ice for 30 min in 50 &, Cells were incubated on ice for 30 min, Cells were then dried and embedded in paraffin, FITC-conjugated antibody to mouse IgG was added, Method: 10 ug of protein was applied to the gel, Method: 3 days after transfection of cells with the indicated constructs, Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for human APRIL, Samples were rehydrated by washes in 90%, Sections were cut, Slides were then successively washed 2x 10min in xylol, This product is for research use only, and incubated for 1 hour with 5 ug/mL Aprily-8 or control mouse IgG (isotype control) in 1%BSA / 1x PBS for 1 hour, and revealed with Aprily-8 (1 ug/mL) and HRP-coupled anti-mouse secondary antibody (1:4, and then treated 10 min in 100% MeOH/ 0, blocked with IgG, cells were fixed with 4% formaldehyde 5 min at RT, immunocytochemistry and Western Blot, mounted on slides and dried overnight at 50 grades C, samples were dehydrated by washes in 60%, samples were incubated with the secondary Ab for 1 hour, slides were washed 3x in PBS, washed and analyzed by flow cytometry, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC, #956, 000), 02% azide) containing 10 ug/mL of Aprily-8 antibody, 6% H202 to inhibit endogenous peroxidase, APRIL antibody can be used to recognize human APRIL by Flow Cytometry, Note: The hAPRIL construct used in this experiment is an uncleavable fusion protein between hBAFF (aa 1-132) and hAPRIL (aa 93-233), l FACS buffer (PBS |
| Suspention, pH, azide: |
02% sodium azide, PBS containing 0 |
| Keep at: |
stable for one year, APRIL antibody can be stored at 4 grades C |
| Omim nr: |
13124605 |
| Ncbi nr: |
O75888 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
8741 |
| GENE symbol: |
TNFSF13 |
| Antigene name: |
member 13, tumor necrosis factor (ligand) superfamily |
| Source: |
Homo sapiens |
| Protein nr: |
O75888 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
APRIL [Aprily-8] |
| Short name: |
anti-APRIL Antibody [Aprily-8] |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-APRIL (Antibody to) [Aprily-8] |
| Alternative technique: |
antibodies |
| Identity: |
11928 |
| Gene: |
TNFSF13 |
More about : TNFSF13 |
| Long gene name: |
tumor necrosis factor superfamily member 13 |
| Synonyms gene name: |
member 13 , tumor necrosis factor (ligand) superfamily |
| Synonyms: |
APRIL CD256 |
| Locus: |
17p13, 1 |
| Discovery year: |
1998-12-04 |
| GenBank acession: |
AF046888 |
| Entrez gene record: |
8741 |
| Pubmed identfication: |
9743536 |
| RefSeq identity: |
NM_003808 |
| Classification: |
Tumor necrosis factor superfamily CD molecules |
| Havana BLAST/BLAT: |
OTTHUMG00000108145 |