| Gene names: |
A proliferation-inducing ligand, APRIL, CD256, TALL-2, TALL2, TRDL-1, Tumor necrosis factor ligand superfamily member 13, UNQ383/PRO715, ZTNF2, APRIL |
| Antibodie's label: |
unlabelled serum |
| Purity: |
=95% in SDS-PAGE, APRIL (monoclonal Aprily-1) antibody was purified to > |
| Ab raised in: |
Mouse |
| Type: |
Monoclonal |
| Monoclonal: |
Aprily-1 |
| Immunoglobulin: |
IgG1 |
| Antigen: |
93-233), APRIL (monoclonal Aprily-1) antibody was made against recombinant human APRIL (aa |
| Immuno application: |
FLOW, Western Blot |
| Antibody advice: |
0, 0, 5% Fetal calf serum, 5% Fetal calf serum, After washing in FACS buffer, After washing in FACS buffer, Anti-APRIL (MAb Aprily-1) does not cross react with mouse APRIL, Beads were washed twice with PBS, Cells (5x105) were incubated on ice for 30 min in 50 &, Cells were incubated on ice for 30 min, Cells were incubated on ice for 30 min, Cells were incubated on ice for 30 min in 50 &, FITC-conjugated antibody to mouse IgG was added, Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mDR6 (thick line), Method: Cell supernatants were first precleared with protein-A sepharose and then subjected to immunoprecipitation with rhBCMA:Fc and protein-A sepharose, Method: HEK 293 , PE-conjugated antibody to rat IgG was added, neutralized with Tris buffer and run on an SDS-PAGE gel and analyzed with 2 ug/mL Aprily-1 by Western blot, rhsMegaAPRIL , washed and analyzed by flow cytometry, washed and then analyzed by flow cytometry, #956, #956, , , 02% azide) containing 1 ug/mL of Luke-1 antibody, 02% azide) containing 10 ug/mL of Aprily-1 antibody, 5 for 10 min, 5mM glycine pH 2, APRIL antibody can be used to recognize human APRIL by Flow Cytometry (also works for intracellular FC staining) and Western Blot, Immunoprecipitated materials were eluted with 2, Note: In some migrations endogenous soluble APRIL resolved as a doublet around 20kDa (dendritic cells: DC), cells were mock transfected or transfected with an expression plasmid enabling surface expression of hAPRIL, l FACS buffer (PBS, l FACS buffer (PBS, served as a positive control |
| Suspention, pH, azide: |
02% sodium azide, PBS containing 0 |
| Keep at: |
stable for one year, APRIL antibody can be stored at 4 grades C |
| Omim nr: |
13124605 |
| Ncbi nr: |
O75888 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
8741 |
| GENE symbol: |
TNFSF13 |
| Antigene name: |
member 13, tumor necrosis factor (ligand) superfamily |
| Source: |
Homo sapiens |
| Protein nr: |
O75888 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
APRIL [Aprily-1] |
| Short name: |
anti-APRIL Antibody [Aprily-1] |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-APRIL (Antibody to) [Aprily-1] |
| Alternative technique: |
antibodies |
| Identity: |
11928 |
| Gene: |
TNFSF13 |
More about : TNFSF13 |
| Long gene name: |
tumor necrosis factor superfamily member 13 |
| Synonyms gene name: |
member 13 , tumor necrosis factor (ligand) superfamily |
| Synonyms: |
APRIL CD256 |
| Locus: |
17p13, 1 |
| Discovery year: |
1998-12-04 |
| GenBank acession: |
AF046888 |
| Entrez gene record: |
8741 |
| Pubmed identfication: |
9743536 |
| RefSeq identity: |
NM_003808 |
| Classification: |
Tumor necrosis factor superfamily CD molecules |
| Havana BLAST/BLAT: |
OTTHUMG00000108145 |