| Gene names: |
C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, GRB, Granzyme B, HLP, SECT, CGL1 |
| Antibodie's label: |
FITC |
| Purity: |
#8805, 1) antibody was purified to &, 95% in SDS-PAGE, Granzyme B (monoclonal B18 |
| Ab raised in: |
Mouse |
| Type: |
Monoclonal |
| Monoclonal: |
1, B18 |
| Immunoglobulin: |
IgG2a |
| Antigen: |
amino acids 7-227, 1) antibody was made against recombinant human granzyme B, Granzyme B (monoclonal B18 |
| Immuno application: |
immuno precipitation, immuno-cyto-chemistry, immuno-histo-chemistry, Western Blot |
| Antibody advice: |
0, 0, 0, 5% Fetal calf serum, After 3 washes in PBS, After rinsing in PBS, After washing in FACS buffer, Method: : Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, Method: Human T cells were plated onto polylysine treated glass slides, Method: Permeabilized human T cells (5 x 105) were incubated on ice for 30 min in 50 &, Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system, a FITC-conjugated anti-mouse IgG was added for 30 min, cells were analyzed by flow cytometry, fixed and permeabilized in methanol at -20oC for 5 min, immunocytochemistry, immunohistochemistry (frozen sections, immunoprecipitation and western blot, paraffin sections), slides washed again in PBS and visualized using a fluorescence microscope, slides were incubated for 1 hr at room temperature (RT) with 20 ug/mL of B18, then in acetone at -20oC for 30 sec, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18, #956, 02% azide) containing 1 ug of FITC- labeled B18, 1 antibody, 1 at 1 ug/mL, 1% BSA, 1% BSA, 4 antibody in PBS, Granzyme B antibody can be used for detection of human granzyme B by flow cytometry, l FACS buffer (PBS |
| Suspention, pH, azide: |
02% sodium azide, PBS containing 0 |
| Keep at: |
stable for one year, Granzyme B antibody can be stored at 4 grades C |
| Omim nr: |
317373361 |
| Ncbi nr: |
P10144 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
3002 |
| GENE symbol: |
GZMB |
| Antigene name: |
cytotoxic T-lymphocyte-associated serine esterase 1), granzyme B (granzyme 2 |
| Source: |
Homo sapiens |
| Protein nr: |
P10144 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
1], Granzyme B [B18 |
| Short name: |
1], anti-Granzyme B Antibody [B18 |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
1], antibody to-Granzyme B (Antibody to) [B18 |
| Alternative technique: |
antibodies |