| Antigen detectable with this ELISA kit: |
Vinculin (VCL) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1250pg/mL, 156, 2500pg/mL, 312, 625pg/mL, 78, 125-5000pg/mL The standard curve concentrations used for the ELISA&rsquo, 125pg/mL, 25pg/mL, 5pg/mL, 78, s were 5000pg/mL |
| Sensitivity: |
The minimum detectable dose of this kit is typically less than 33pg/mL |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vinculin (VCL), The concentration of Vinculin (VCL) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vinculin (VCL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Vinculin (VCL), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Vinculin (VCL) |
| Short name: |
Vinculin (VCL) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Vinculin (vinculin) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CMD1W and CMH15 and HEL114 and MV and MVCL, Extracellular, VCL and IDBG-630608 and ENSBTAG00000021879 and 783645, VCL and IDBG-78928 and ENSG00000035403 and 7414, Vcl and IDBG-136687 and ENSMUSG00000021823 and 22330, actin filament binding, this GO :0002009 and morphogenesis of an epithelium and biological process this GO :0002162 and dystroglycan binding and molecular function this GO :0002576 and platelet degranulation and biological process this GO :0003779 and actin binding and molecular function this GO :0005198 and structural molecule activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005829 and cytosol and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0005912 and adherens junction and cellular component this GO :0005913 and cell-cell adherens junction and cellular component this GO :0005916 and fascia adherens and cellular component this GO :0005925 and focal adhesion and cellular component this GO :0006928 and cellular component movement and biological process this GO :0006936 and muscle contraction and biological process this GO :0007155 and cell adhesion and biological process this GO :0007160 and cell-matrix adhesion and biological process this GO :0007596 and blood coagulation and biological process this GO :0008013 and beta-catenin binding and molecular function this GO :0015629 and actin cytoskeleton and cellular component this GO :0030032 and lamellipodium assembly and biological process this GO :0030055 and cell-substrate junction and cellular component this GO :0030168 and platelet activation and biological process this GO :0030334 and regulation of cell migration and biological process this GO :0030336 and negative regulation of cell migration and biological process this GO :0034333 and adherens junction assembly and biological process this GO :0034394 and protein localization to cell surface and biological process this GO :0043034 and costamere and cellular component this GO :0043234 and protein complex and cellular component this GO :0043297 and apical junction assembly and biological process this GO :0045294 and alpha-catenin binding and molecular function this GO :0045296 and cadherin binding and molecular function this GO :0051015 and actin filament binding and molecular function this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0090136 and epithelial cell-cell adhesion and biological process, this GO :0002162 : dystroglycan binding, this GO :0002162 : dystroglycan binding and also this GO :0003779 : actin binding and also this GO :0005198 : structural molecule activity and also this GO :0005515 : protein binding and also this GO :0008013 : beta-catenin binding and also this GO :0045294 : alpha-catenin binding and also this GO :0045296 : cadherin binding and also this GO :0051015 : actin filament binding, this GO :0003779 : actin binding, this GO :0005198 : structural molecule activity, this GO :0005515 : protein binding, this GO :0008013 : beta-catenin binding, this GO :0045294 : alpha-catenin binding, this GO :0045296 : cadherin binding, this GO :0051015 : actin filament binding, vinculin |
| Identity: |
12665 |
| Gene: |
VCL |
More about : VCL |
| Long gene name: |
vinculin |
| Synonyms name: |
metavinculin |
| Locus: |
10q22, 2 |
| Discovery year: |
1990-09-10 |
| GenBank acession: |
M33308 |
| Entrez gene record: |
7414 |
| Pubmed identfication: |
1339348 |
| RefSeq identity: |
NM_003373 NM_014000 |
| Havana BLAST/BLAT: |
OTTHUMG00000018498 |
| Locus Specific Databases: |
LRG_383 |