| We send this reagant on: |
Blue Ice |
| The temperatureerature for storage is: |
-20º, C |
| SDS or safty data sheet: |
more (VPK-410 through VPK-430-SER6) GHS, pdf |
| Type of reagent: |
Viral Expression |
| Related techniques: |
AAV Expression and Packaging, AAV Helper Free Complete Expression Systems, AAV-DJ Helper Free Complete Expression Systems, Adeno-Associated Virus (AAV) Expression, Viral Expression |
| Use of the reagent: |
AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, AAV-4, AAV-5, AAV-8, AAV-9, E4 and VA RNA) or in the 293 packaging cells (E1), The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2, and caprine AAV, as well as the novel AAV-DJ and AAV-DJ/8, avian AAV, bovine AAV, The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus |
| Cellular use: |
expression vector and GFP control vector, Complete Expression System contains packaging plasmids |
| Protocol information: |
E4, and VA RNA adenoviral genes, eliminates the need for a helper adenovirus, pHelper plasmid contains required E2A |
| Reagent properties: |
AAV-DJ system provides a hybrid capsid created from 8 different AAV serotypes to provide substantially higher infectivity rates across a broad range of tissues |
| Additional lab advice: |
9 kb, Cloning capacity of expression vector = 3 |
| Living modified organisme in this reagent or LMO: |
Not applicable |
| Species compatible to: |
Humans |
| Use of this test kit or product: |
Genetics lab |
| Antigen: |
a specific protein sequence |
| Buffer: |
05, The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7 |
| Molecular weight: |
in kilo Danlto or kDa |
| This reagent is purified: |
97 |
| Gene target: |
AAV-DJ Helper Free Promoterless Expression |
| Short name: |
AAV-DJ Helper Free Promoterless Expression System |
| Alternative name: |
AAV-DJ Helper Free Promoterless Expression System |