| Antigen detectable with this ELISA kit: |
Mitochondrial (UCP1), Uncoupling Protein 1 |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
122ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Mitochondrial (UCP1), Mitochondrial (UCP1), Mitochondrial (UCP1), Mitochondrial (UCP1) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uncoupling Protein 1, The concentration of Uncoupling Protein 1, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uncoupling Protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Uncoupling Protein 1, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Mitochondrial (UCP1), Uncoupling Protein 1 |
| Short name: |
Mitochondrial (UCP1) ELISA kit, Uncoupling Protein 1 |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Mitochondrial (uncoupling protein 1 (mitochondrial, proton carrier)) Enzyme-linked immunosorbent assay reagent, Uncoupling Protein 1 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Plasma membranes, SLC25A7 and UCP, UCP1 and IDBG-38859 and ENSG00000109424 and 7350, UCP1 and IDBG-629680 and ENSBTAG00000004647 and 101910176, Ucp1 and IDBG-171838 and ENSMUSG00000031710 and 22227, oxidative phosphorylation uncoupler activity, proton carrier), this GO :0005515 and protein binding and molecular function this GO :0005739 and mitochondrion and cellular component this GO :0005740 and mitochondrial envelope and cellular component this GO :0005743 and mitochondrial inner membrane and cellular component this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0006839 and mitochondrial transport and biological process this GO :0015992 and proton transport and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0017077 and oxidative phosphorylation uncoupler activity and molecular function this GO :0022904 and respiratory electron transport chain and biological process this GO :0044237 and cellular metabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0050873 and brown fat cell differentiation and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0017077 : oxidative phosphorylation uncoupler activity, this GO :0017077 : oxidative phosphorylation uncoupler activity, 281561, uncoupling protein 1 (mitochondrial |
| Identity: |
12517 |
| Gene: |
UCP1 |
More about : UCP1 |
| Long gene name: |
uncoupling protein 1 |
| Synonyms gene: |
UCP |
| Synonyms gene name: |
proton carrier) , uncoupling protein 1 (mitochondrial |
| Synonyms: |
SLC25A7 |
| Locus: |
4q31, 1 |
| Discovery year: |
1991-08-19 |
| GenBank acession: |
X51955 |
| Entrez gene record: |
7350 |
| Pubmed identfication: |
2380264 |
| Classification: |
Solute carriers |
| Havana BLAST/BLAT: |
OTTHUMG00000133415 |