| Category: |
Antibody |
| Concentration: |
05% sodium azide, 1 mg/ml BSA (US sourced) and 0, 2 mg/ml in 1X PBS with 0 |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
IDH |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
, Due to limited knowledge and inability to test the antibody against all known species, Other species not tested, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IF, WB, FACS |
| Recommended dilutions: |
5-1ug/million cells, 5-1ug/ml, 5-1ug/ml, Flow Cytometry: 0, Immunofluorescence: 0, Western blot: 0 |
| Notes: |
Optimal dilution of the IDH antibody should be determined by the researcher |
| Intented use: |
This IDH antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O75874 |
| Purity: |
Purified by protein A Affinity Chromatography |
| Description: |
During this process, IDH appears to function as a tumor suppressor that, IDH catalyzes the third step of the citric acid cycle, It belongs to the isocitrate and isopropylmalate dehydrogenases family, Recently, The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, an inactivating mutation of IDH has been implicated in glioblastoma, contributes to tumorigenesis in part through induction of the HIF-1 pathway, either NADH or NADPH is produced along with CO2, forming a-ketoglutarate and CO2 in a two-step reaction, when mutationally inactivated, which involves the oxidative decarboxylation of isocitrate, which is identified as isocitrate dehydrogenase (IDH1), while the second step involves the production of a-ketoglutarate, It recognizes a 45kDa protein |
| Immunogen: |
aa 296-414) from the human protein was used as the immunogen for the IDH antibody, A recombinant fragment (119 amino acid residues |
| Storage: |
Store the IDH antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide) |
| Localization: |
nuclear, Cytoplasmic & |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
IDH |
| Short name: |
IDH Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
IDH (Antibody to) |
| Alternative technique: |
antibodies |