| Antigen detectable with this ELISA kit: |
Member 12 (TNFSF12), Tumor Necrosis Factor Ligand Superfamily |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
8pg/mL, The minimum detectable dose of this kit is typically less than 5 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Member 12 (TNFSF12), Member 12 (TNFSF12), Member 12 (TNFSF12), Member 12 (TNFSF12) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Ligand Superfamily, The concentration of Tumor Necrosis Factor Ligand Superfamily, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Tumor Necrosis Factor Ligand Superfamily, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description: |
Receptor agonists are often tested for drug development, FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling |
| Gene target: |
Member 12 (TNFSF12), Tumor Necrosis Factor Ligand Superfamily |
| Short name: |
Member 12 (TNFSF12) ELISA kit, Tumor Necrosis Factor Ligand Superfamily |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Member 12 (tumor necrosis factor (ligand) superfamily, member 12) Enzyme-linked immunosorbent assay reagent, Tumor Necrosis Factor Ligand supergroup |
| Alternative technique: |
elisas |
| Alternative to gene target: |
APO3L and DR3LG and TWEAK, Extracellular, TNFSF12 and IDBG-408330 and ENSG00000239697 and 8742, TNFSF12 and IDBG-634998 and ENSBTAG00000031725 and 100551513, Tnfsf12 and IDBG-708803 and ENSMUSG00000097328 and 21944, member 12, protein binding, this GO :0001525 and angiogenesis and biological process this GO :0001938 and positive regulation of endothelial cell proliferation and biological process this GO :0005102 and receptor binding and molecular function this GO :0005125 and cytokine activity and molecular function this GO :0005164 and tumor necrosis factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006915 and apoptotic process and biological process this GO :0006955 and immune response and biological process this GO :0007165 and signal transduction and biological process this GO :0016020 and membrane and cellular component this GO :0030154 and cell differentiation and biological process this GO :0043542 and endothelial cell migration and biological process this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0097190 and apoptotic signaling pathway and biological process this GO :2001238 and positive regulation of extrinsic apoptotic signaling pathway and biological process, this GO :0005102 : receptor binding, this GO :0005102 : receptor binding and also this GO :0005125 : cytokine activity and also this GO :0005164 : tumor necrosis factor receptor binding and also this GO :0005515 : protein binding, this GO :0005125 : cytokine activity, this GO :0005164 : tumor necrosis factor receptor binding, this GO :0005515 : protein binding, 517833, 788103, tumor necrosis factor (ligand) superfamily |
| Identity: |
11927 |
| Gene: |
TNFSF12 |
More about : TNFSF12 |
| Long gene name: |
tumor necrosis factor superfamily member 12 |
| Synonyms gene name: |
member 12 , tumor necrosis factor (ligand) superfamily |
| Synonyms: |
TWEAK DR3LG APO3L |
| Locus: |
17p13, 1 |
| Discovery year: |
1998-12-04 |
| GenBank acession: |
AF030099 |
| Entrez gene record: |
8742 |
| Pubmed identfication: |
9405449 9560343 |
| RefSeq identity: |
NM_003809 |
| Classification: |
Tumor necrosis factor superfamily |
| Havana BLAST/BLAT: |
OTTHUMG00000108148 |