| Antigen detectable with this ELISA kit: |
Toll Like Receptor 5 (TLR5) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
135ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 5 (TLR5), The concentration of Toll Like Receptor 5 (TLR5) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 5 (TLR5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Toll Like Receptor 5 (TLR5), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Toll Like Receptor 5 (TLR5) |
| Short name: |
Toll Like Receptor 5 (TLR5) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Toll Like Receptor 5 (toll-like receptor 5) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
MELIOS and SLE1 and SLEB1 and TIL3, Plasma membranes, TLR5 and IDBG-106840 and ENSG00000187554 and 7100, TLR5 and IDBG-648348 and ENSBTAG00000000477 and 444870, Tlr5 and IDBG-259066 and ENSMUSG00000079164 and 53791, protein binding, this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005149 and interleukin-1 receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0008584 and male this GO nad development and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0032757 and positive regulation of interleukin-8 production and biological process this GO :0034123 and positive regulation of toll-like receptor signaling pathway and biological process this GO :0034146 and toll-like receptor 5 signaling pathway and biological process this GO :0034166 and toll-like receptor 10 signaling pathway and biological process this GO :0042742 and defense response to bacterium and biological process this GO :0045087 and innate immune response and biological process this GO :0045429 and positive regulation of nitric oxide biosynthetic process and biological process this GO :0050707 and regulation of cytokine secretion and biological process this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process, this GO :0004888 : transmembrane signaling receptor activity, this GO :0004888 : transmembrane signaling receptor activity and also this GO :0005149 : interleukin-1 receptor binding and also this GO :0005515 : protein binding, this GO :0005149 : interleukin-1 receptor binding, this GO :0005515 : protein binding, toll-like receptor 5 |
| Identity: |
11851 |
| Gene: |
TLR5 |
More about : TLR5 |
| Long gene name: |
toll like receptor 5 |
| Synonyms gene: |
SLEB1 |
| Synonyms gene name: |
systemic lupus erythematosus susceptibility 1 toll-like receptor 5 |
| Synonyms: |
TIL3 FLJ10052 MGC126430 MGC126431 |
| Synonyms name: |
Toll/interleukin-1 receptor-like protein 3 |
| Locus: |
1q41 |
| Discovery year: |
1998-06-25 |
| Entrez gene record: |
7100 |
| Pubmed identfication: |
9435236 16027372 |
| RefSeq identity: |
NM_003268 |
| Classification: |
Toll like receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000037937 |