| Antigen detectable with this ELISA kit: |
Semaphorin 7A (SEMA7A) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
108ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 7A (SEMA7A), The concentration of Semaphorin 7A (SEMA7A) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 7A (SEMA7A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Semaphorin 7A (SEMA7A), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Semaphorin 7A (SEMA7A) |
| Short name: |
Semaphorin 7A (SEMA7A) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
glucose-6-phosphate isomerase membrane anchor (John Milton Hagen blood family)) Enzyme-linked immunosorbent assay reagent, Semaphorin 7A (semaphorin 7A |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CD108 and CDw108 and H-SEMA-K1 and H-Sema-L and JMH and SEMAK1 and SEMAL, Cell surfaces, GPI membrane anchor (John Milton Hagen blood group), SEMA7A and IDBG-22070 and ENSG00000138623 and 8482, SEMA7A and IDBG-631814 and ENSBTAG00000011446 and 614707, Sema7a and IDBG-171171 and ENSMUSG00000038264 and 20361, protein binding, this GO :0001649 and osteoblast differentiation and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0004872 and receptor activity and molecular function this GO :0005178 and integrin binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0006954 and inflammatory response and biological process this GO :0006955 and immune response and biological process this GO :0007229 and integrin-mediated signaling pathway and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007411 and axon guidance and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0016020 and membrane and cellular component this GO :0021988 and olfactory lobe development and biological process this GO :0031175 and neuron projection development and biological process this GO :0031225 and anchored component of membrane and cellular component this GO :0045773 and positive regulation of axon extension and biological process this GO :0048675 and axon extension and biological process this GO :0050727 and regulation of inflammatory response and biological process this GO :0060907 and positive regulation of macrophage cytokine production and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0005178 : integrin binding and also this GO :0005515 : protein binding, this GO :0005178 : integrin binding, this GO :0005515 : protein binding, semaphorin 7A |
| Identity: |
10741 |
| Gene: |
SEMA7A |
More about : SEMA7A |
| Long gene name: |
semaphorin 7A (John Milton Hagen blood group) |
| Synonyms gene: |
SEMAL |
| Synonyms gene name: |
(semaphorin) 7A (JMH blood group) semaphorin 7A, (semaphorin) 7A sema domain, GPI membrane anchor (John Milton Hagen blood group) , and GPI membrane anchor, and GPI membrane anchor, immunoglobulin domain (Ig), immunoglobulin domain (Ig), sema domain |
| Synonyms: |
H-Sema-L CD108 |
| Synonyms name: |
John Milton Hagen blood group H-Sema K1 |
| Locus: |
15q24, 1 |
| Discovery year: |
1999-06-25 |
| GenBank acession: |
AF069493 |
| Entrez gene record: |
8482 |
| Pubmed identfication: |
9721204 |
| RefSeq identity: |
NM_003612 |
| Classification: |
CD molecules Semaphorins Immunoglobulin like domain containing Blood group antigens |
| Havana BLAST/BLAT: |
OTTHUMG00000139000 |
| Locus Specific Databases: |
LRG_818 |