| Antigen detectable with this ELISA kit: |
Semaphorin 5A (SEMA5A) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 1, 25ng/mL, 3, 6, 12, 125ng/mL, 25ng/mL, 563ng/mL, 5ng/mL, 781-50ng/mL The standard curve concentrations used for the ELISA&rsquo, 781ng/mL, s were 50ng/mL |
| Sensitivity: |
27ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 5A (SEMA5A), The concentration of Semaphorin 5A (SEMA5A) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 5A (SEMA5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Semaphorin 5A (SEMA5A), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Semaphorin 5A (SEMA5A) |
| Short name: |
Semaphorin 5A (SEMA5A) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
(semaphorin) 5A) Enzyme-linked immunosorbent assay reagent, seven thrombospondin repeats (classification 1 and classification 1-like), transmembrane domain (TM) and short cytoplasmic domain, Semaphorin 5A (sema domain |
| Alternative technique: |
elisas |
| Alternative to gene target: |
(semaphorin) 5A, Plasma membranes, SEMA5A and IDBG-12173 and ENSG00000112902 and 9037, SEMA5A and IDBG-631754 and ENSBTAG00000005021 and 506636, SEMAF and semF, Sema5a and IDBG-133049 and ENSMUSG00000022231 and 20356, seven thrombospondin repeats (type 1 and type 1-like), syndecan binding, this GO :0001569 and patterning of blood vessels and biological process this GO :0001938 and positive regulation of endothelial cell proliferation and biological process this GO :0002043 and blood vessel endothelial cell proliferation involved in sprouting angiogenesis and biological process this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0007162 and negative regulation of cell adhesion and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007399 and nervous system development and biological process this GO :0007411 and axon guidance and biological process this GO :0007413 and axonal fasciculation and biological process this GO :0008046 and axon guidance receptor activity and molecular function this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0021536 and diencephalon development and biological process this GO :0030215 and semaphorin receptor binding and molecular function this GO :0030836 and positive regulation of actin filament depolymerization and biological process this GO :0035373 and chondroitin sulfate proteoglycan binding and molecular function this GO :0035413 and positive regulation of catenin import into nucleus and biological process this GO :0043395 and heparan sulfate proteoglycan binding and molecular function this GO :0045545 and syndecan binding and molecular function this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0048754 and branching morphogenesis of an epithelial tube and biological process this GO :0048842 and positive regulation of axon extension involved in axon guidance and biological process this GO :0048843 and negative regulation of axon extension involved in axon guidance and biological process this GO :0050918 and positive chemotaxis and biological process this GO :0051897 and positive regulation of protein kinase B signaling and biological process this GO :0060326 and cell chemotaxis and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :1990256 and signal clustering and biological process this GO :2000352 and negative regulation of endothelial cell apoptotic process and biological process this GO :2001028 and positive regulation of endothelial cell chemotaxis and biological process, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0005515 : protein binding and also this GO :0008046 : axon guidance receptor activity and also this GO :0030215 : semaphorin receptor binding and also this GO :0035373 : chondroitin sulfate proteoglycan binding and also this GO :0043395 : heparan sulfate proteoglycan binding and also this GO :0045545 : syndecan binding, this GO :0005515 : protein binding, this GO :0008046 : axon guidance receptor activity, this GO :0030215 : semaphorin receptor binding, this GO :0035373 : chondroitin sulfate proteoglycan binding, this GO :0043395 : heparan sulfate proteoglycan binding, this GO :0045545 : syndecan binding, transmembrane domain (TM) and short cytoplasmic domain, sema domain |
| Identity: |
10736 |
| Gene: |
SEMA5A |
More about : SEMA5A |
| Long gene name: |
semaphorin 5A |
| Synonyms gene: |
SEMAF |
| Synonyms gene name: |
(semaphorin) 5A , sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain |
| Synonyms: |
semF |
| Locus: |
5p15, 31 |
| Discovery year: |
1999-06-25 |
| GenBank acession: |
U52840 |
| Entrez gene record: |
9037 |
| Pubmed identfication: |
8817451 9464278 |
| Classification: |
Semaphorins |
| Havana BLAST/BLAT: |
OTTHUMG00000090501 |