| Antigen detectable with this ELISA kit: |
Semaphorin 4B (SEMA4B) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
071ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4B (SEMA4B), The concentration of Semaphorin 4B (SEMA4B) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4B (SEMA4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Semaphorin 4B (SEMA4B), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Semaphorin 4B (SEMA4B) |
| Short name: |
Semaphorin 4B (SEMA4B) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
(semaphorin) 4B) Enzyme-linked immunosorbent assay reagent, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, Semaphorin 4B (sema domain |
| Alternative technique: |
elisas |
| Alternative to gene target: |
(semaphorin) 4B, Plasma membranes, SEMA4B and IDBG-29866 and ENSG00000185033 and 10509, SEMA4B and IDBG-629265 and ENSBTAG00000021273 and 510140, SEMAC and SemC, Sema4b and IDBG-194649 and ENSMUSG00000030539 and 20352, immunoglobulin domain (Ig), protein binding, this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0007275 and multicellular organismal development and biological process this GO :0007399 and nervous system development and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0030154 and cell differentiation and biological process this GO :0045202 and synapse and cellular component, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, transmembrane domain (TM) and short cytoplasmic domain, sema domain |
| Identity: |
10730 |
| Gene: |
SEMA4B |
More about : SEMA4B |
| Long gene name: |
semaphorin 4B |
| Synonyms gene: |
SEMAC |
| Synonyms gene name: |
(semaphorin) 4B , immunoglobulin domain (Ig), sema domain, transmembrane domain (TM) and short cytoplasmic domain |
| Synonyms: |
SemC KIAA1745 MGC131831 |
| Locus: |
15q25 |
| Discovery year: |
1999-06-25 |
| GenBank acession: |
AB051532 |
| Entrez gene record: |
10509 |
| Pubmed identfication: |
7748561 |
| RefSeq identity: |
NM_198925 |
| Classification: |
Semaphorins |
| Havana BLAST/BLAT: |
OTTHUMG00000172096 |