| Antigen detectable with this ELISA kit: |
Semaphorin 3A (SEMA3A) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
1pg/mL, The minimum detectable dose of this kit is typically less than 6 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 3A (SEMA3A), The concentration of Semaphorin 3A (SEMA3A) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3A (SEMA3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Semaphorin 3A (SEMA3A), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Semaphorin 3A (SEMA3A) |
| Short name: |
Semaphorin 3A (SEMA3A) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
(semaphorin) 3A) Enzyme-linked immunosorbent assay reagent, immunoglobulin domain (Ig), secreted, short basic domain, Semaphorin 3A (sema domain |
| Alternative technique: |
elisas |
| Alternative to gene target: |
(semaphorin) 3A, Extracellular, SEMA3A and IDBG-24546 and ENSG00000075213 and 10371, SEMA3A and IDBG-630984 and ENSBTAG00000018133 and 537777, Sema3a and IDBG-135492 and ENSMUSG00000028883 and 20346, chemorepellent activity, coll-1 and COLL1 and HH16 and Hsema-I and Hsema-III and SEMA1 and SEMAD and SEMAIII and SEMAL and SemD, immunoglobulin domain (Ig), secreted, short basic domain, this GO :0001764 and neuron migration and biological process this GO :0002027 and regulation of heart rate and biological process this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0006915 and apoptotic process and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007411 and axon guidance and biological process this GO :0007413 and axonal fasciculation and biological process this GO :0008045 and motor neuron axon guidance and biological process this GO :0010633 and negative regulation of epithelial cell migration and biological process this GO :0016020 and membrane and cellular component this GO :0021612 and facial nerve structural organization and biological process this GO :0021637 and trigeminal nerve structural organization and biological process this GO :0021675 and nerve development and biological process this GO :0021772 and olfactory bulb development and biological process this GO :0021785 and branchiomotor neuron axon guidance and biological process this GO :0021828 and this GO nadotrophin-releasing hormone neuronal migration to the hypothalamus and biological process this GO :0030215 and semaphorin receptor binding and molecular function this GO :0030424 and axon and cellular component this GO :0030425 and dendrite and cellular component this GO :0030517 and negative regulation of axon extension and biological process this GO :0038191 and neuropilin binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045499 and chemorepellent activity and molecular function this GO :0048485 and sympathetic nervous system development and biological process this GO :0048813 and dendrite morphogenesis and biological process this GO :0048841 and regulation of axon extension involved in axon guidance and biological process this GO :0048843 and negative regulation of axon extension involved in axon guidance and biological process this GO :0048846 and axon extension involved in axon guidance and biological process this GO :0048880 and sensory system development and biological process this GO :0050919 and negative chemotaxis and biological process this GO :0060385 and axonogenesis involved in innervation and biological process this GO :0060666 and dichotomous subdivision of terminal units involved in salivary gland branching and biological process this GO :0061549 and sympathetic ganglion development and biological process this GO :0071526 and semaphorin-plexin signaling pathway and biological process this GO :0097490 and sympathetic neuron projection extension and biological process this GO :0097491 and sympathetic neuron projection guidance and biological process this GO :1901166 and neural crest cell migration involved in autonomic nervous system development and biological process this GO :1902285 and semaphorin-plexin signaling pathway involved in neuron projection guidance and biological process this GO :1902287 and semaphorin-plexin signaling pathway involved in axon guidance and biological process this GO :1903045 and neural crest cell migration involved in sympathetic nervous system development and biological process this GO :2000020 and positive regulation of male this GO nad development and biological process this GO :2001224 and positive regulation of neuron migration and biological process, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0005515 : protein binding and also this GO :0030215 : semaphorin receptor binding and also this GO :0038191 : neuropilin binding and also this GO :0045499 : chemorepellent activity, this GO :0005515 : protein binding, this GO :0030215 : semaphorin receptor binding, this GO :0038191 : neuropilin binding, this GO :0045499 : chemorepellent activity, sema domain |
| Identity: |
10723 |
| Gene: |
SEMA3A |
More about : SEMA3A |
| Long gene name: |
semaphorin 3A |
| Synonyms gene: |
SEMAD |
| Synonyms gene name: |
(semaphorin) 3A , immunoglobulin domain (Ig), secreted, sema domain, short basic domain |
| Synonyms: |
SEMA1 SemD coll-1 Hsema-I |
| Synonyms name: |
sema III |
| Locus: |
7q21, 11 |
| Discovery year: |
1999-06-25 |
| GenBank acession: |
L26081 |
| Entrez gene record: |
10371 |
| Pubmed identfication: |
8269517 7748561 |
| RefSeq identity: |
NM_006080 |
| Classification: |
Semaphorins Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000023443 |