| Antigen detectable with this ELISA kit: |
Endothelium (SELE), Selectin |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1250pg/mL, 39, 39, 78, 156, 312, 625pg/mL, 063-2500pg/mL The standard curve concentrations used for the ELISA&rsquo, 063pg/mL, 125pg/mL, 25pg/mL, 5pg/mL, s were 2500pg/mL |
| Sensitivity: |
The minimum detectable dose of this kit is typically less than 14pg/mL |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Endothelium (SELE), Endothelium (SELE), Endothelium (SELE), Endothelium (SELE) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Selectin, The concentration of Selectin, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Selectin, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Endothelium (SELE), Selectin |
| Short name: |
Endothelium (SELE) ELISA kit, Selectin |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Endothelium (selectin E) Enzyme-linked immunosorbent assay reagent, Selectin |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CD62E and ELAM and ELAM1 and ESEL and LECAM2, Extracellular, SELE and IDBG-104701 and ENSG00000007908 and 6401, SELE and IDBG-631886 and ENSBTAG00000007307 and 281484, Sele and IDBG-202283 and ENSMUSG00000026582 and 20339, oli this GO saccharide binding, this GO :0002092 and positive regulation of receptor internalization and biological process this GO :0002523 and leukocyte migration involved in inflammatory response and biological process this GO :0002687 and positive regulation of leukocyte migration and biological process this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005901 and caveola and cellular component this GO :0005905 and coated pit and cellular component this GO :0006954 and inflammatory response and biological process this GO :0007155 and cell adhesion and biological process this GO :0007157 and heterophilic cell-cell adhesion and biological process this GO :0007159 and leukocyte cell-cell adhesion and biological process this GO :0007202 and activation of phospholipase C activity and biological process this GO :0007596 and blood coagulation and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0019722 and calcium-mediated signaling and biological process this GO :0030029 and actin filament-based process and biological process this GO :0030246 and carbohydrate binding and molecular function this GO :0030863 and cortical cytoskeleton and cellular component this GO :0032496 and response to lipopolysaccharide and biological process this GO :0033691 and sialic acid binding and molecular function this GO :0034612 and response to tumor necrosis factor and biological process this GO :0043274 and phospholipase binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050727 and regulation of inflammatory response and biological process this GO :0050900 and leukocyte migration and biological process this GO :0050901 and leukocyte tethering or rolling and biological process this GO :0070492 and oli this GO saccharide binding and molecular function this GO :0070555 and response to interleukin-1 and biological process, this GO :0004888 : transmembrane signaling receptor activity, this GO :0004888 : transmembrane signaling receptor activity and also this GO :0005515 : protein binding and also this GO :0030246 : carbohydrate binding and also this GO :0033691 : sialic acid binding and also this GO :0043274 : phospholipase binding and also this GO :0070492 : oli this GO saccharide binding, this GO :0005515 : protein binding, this GO :0030246 : carbohydrate binding, this GO :0033691 : sialic acid binding, this GO :0043274 : phospholipase binding, this GO :0070492 : oli this GO saccharide binding, selectin E |
| Identity: |
10718 |
| Gene: |
SELE |
More about : SELE |
| Long gene name: |
selectin E |
| Synonyms gene: |
ELAM1 ELAM |
| Synonyms gene name: |
endothelial adhesion molecule 1 |
| Synonyms: |
ESEL CD62E |
| Locus: |
1q24, 2 |
| Discovery year: |
1989-06-30 |
| GenBank acession: |
M30640 |
| Entrez gene record: |
6401 |
| Pubmed identfication: |
1375831 |
| RefSeq identity: |
NM_000450 |
| Classification: |
Sushi domain containing Selectins CD molecules C-type lectin domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000034851 |