| Antigen detectable with this ELISA kit: |
Secreted Frizzled Related Protein 4 (SFRP4) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
1000pg/mL, 125pg/mL, 250pg/mL, 31, 500pg/mL, 62, 25-2000pg/mL The standard curve concentrations used for the ELISA&rsquo, 25pg/mL, 31, 5pg/mL, s were 2000pg/mL |
| Sensitivity: |
5pg/mL, The minimum detectable dose of this kit is typically less than 14 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 4 (SFRP4), The concentration of Secreted Frizzled Related Protein 4 (SFRP4) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 4 (SFRP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Secreted Frizzled Related Protein 4 (SFRP4), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Secreted Frizzled Related Protein 4 (SFRP4) |
| Short name: |
Secreted Frizzled Protein 4 (SFRP4) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Secreted Frizzled Related Protein 4 (secreted frizzled-related protein 4) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
FRP-4 and FRPHE and sFRP-4, SFRP4 and IDBG-12815 and ENSG00000106483 and 6424, SFRP4 and IDBG-632677 and ENSBTAG00000015366 and 518004, Sfrp4 and IDBG-132703 and ENSMUSG00000021319 and 20379, Wnt-activated receptor activity, nuclei, this GO :0001944 and vasculature development and biological process this GO :0002092 and positive regulation of receptor internalization and biological process this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0007420 and brain development and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008406 and this GO nad development and biological process this GO :0009725 and response to hormone and biological process this GO :0009986 and cell surface and cellular component this GO :0010628 and positive regulation of gene expression and biological process this GO :0016055 and Wnt signaling pathway and biological process this GO :0017147 and Wnt-protein binding and molecular function this GO :0022601 and menstrual cycle phase and biological process this GO :0030165 and PDZ domain binding and molecular function this GO :0042813 and Wnt-activated receptor activity and molecular function this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043433 and negative regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0045606 and positive regulation of epidermal cell differentiation and biological process this GO :0046329 and negative regulation of JNK cascade and biological process this GO :0046697 and decidualization and biological process this GO :0055062 and phosphate ion homeostasis and biological process this GO :0060056 and mammary gland involution and biological process this GO :0060429 and epithelium development and biological process this GO :0090090 and negative regulation of canonical Wnt signaling pathway and biological process this GO :0090263 and positive regulation of canonical Wnt signaling pathway and biological process this GO :1902174 and positive regulation of keratinocyte apoptotic process and biological process this GO :2000119 and negative regulation of sodium-dependent phosphate transport and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0017147 : Wnt-protein binding and also this GO :0030165 : PDZ domain binding and also this GO :0042813 : Wnt-activated receptor activity, this GO :0017147 : Wnt-protein binding, this GO :0030165 : PDZ domain binding, this GO :0042813 : Wnt-activated receptor activity, secreted frizzled-related protein 4 |
| Identity: |
10778 |
| Gene: |
SFRP4 |
More about : SFRP4 |
| Long gene name: |
secreted frizzled related protein 4 |
| Synonyms gene name: |
secreted frizzled-related protein 4 |
| Synonyms: |
frpHE FRP-4 FRPHE |
| Locus: |
7p14, 1 |
| Discovery year: |
1998-07-15 |
| GenBank acession: |
AF026692 |
| Entrez gene record: |
6424 |
| Pubmed identfication: |
10211996 |
| RefSeq identity: |
NM_003014 |
| Classification: |
Secreted frizzled-related proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000023026 |