| Antigen detectable with this ELISA kit: |
Secreted Frizzled Related Protein 2 (SFRP2) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 1, 25ng/mL, 3, 6, 12, 125ng/mL, 25ng/mL, 563ng/mL, 5ng/mL, 781-50ng/mL The standard curve concentrations used for the ELISA&rsquo, 781ng/mL, s were 50ng/mL |
| Sensitivity: |
34ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 2 (SFRP2), The concentration of Secreted Frizzled Related Protein 2 (SFRP2) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 2 (SFRP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Secreted Frizzled Related Protein 2 (SFRP2), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Secreted Frizzled Related Protein 2 (SFRP2) |
| Short name: |
Secreted Frizzled Protein 2 (SFRP2) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Secreted Frizzled Related Protein 2 (secreted frizzled-related protein 2) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046546 and development of primary male sexual characteristics and biological process this GO :0048018 and receptor a this GO nist activity and molecular function this GO :0048546 and digestive tract morphogenesis and biological process this GO :0050680 and negative regulation of epithelial cell proliferation and biological process this GO :0050732 and negative regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0051216 and cartilage development and biological process this GO :0060028 and convergent extension involved in axis elongation and biological process this GO :0060349 and bone morphogenesis and biological process this GO :0061056 and sclerotome development and biological process this GO :0061133 and endopeptidase activator activity and molecular function this GO :0061185 and negative regulation of dermatome development and biological process this GO :0071425 and hematopoietic stem cell proliferation and biological process this GO :0071481 and cellular response to X-ray and biological process this GO :0090090 and negative regulation of canonical Wnt signaling pathway and biological process this GO :0090175 and regulation of establishment of planar polarity and biological process this GO :0090179 and planar cell polarity pathway involved in neural tube closure and biological process this GO :0090244 and Wnt signaling pathway involved in somitogenesis and biological process this GO :0090263 and positive regulation of canonical Wnt signaling pathway and biological process this GO :1902042 and negative regulation of extrinsic apoptotic signaling pathway via death domain receptors and biological process this GO :1902230 and negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :2000035 and regulation of stem cell division and biological process this GO :2000041 and negative regulation of planar cell polarity pathway involved in axis elongation and biological process, Extracellular, FRP-2 and SARP1 and SDF-5, SFRP2 and IDBG-41862 and ENSG00000145423 and 6423, SFRP2 and IDBG-629019 and ENSBTAG00000018563 and 510821, Sfrp2 and IDBG-154791 and ENSMUSG00000027996 and 20319, endopeptidase activator activity, this GO :0001569 and patterning of blood vessels and biological process this GO :0001756 and somitogenesis and biological process this GO :0001843 and neural tube closure and biological process this GO :0001944 and vasculature development and biological process this GO :0001968 and fibronectin binding and molecular function this GO :0002063 and chondrocyte development and biological process this GO :0003151 and outflow tract morphogenesis and biological process this GO :0003214 and cardiac left ventricle morphogenesis and biological process this GO :0005178 and integrin binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006915 and apoptotic process and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0007420 and brain development and biological process this GO :0007584 and response to nutrient and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008406 and this GO nad development and biological process this GO :0008584 and male this GO nad development and biological process this GO :0009952 and anterior/posterior pattern specification and biological process this GO :0010577 and metalloenzyme activator activity and molecular function this GO :0010629 and negative regulation of gene expression and biological process this GO :0010667 and negative regulation of cardiac muscle cell apoptotic process and biological process this GO :0010719 and negative regulation of epithelial to mesenchymal transition and biological process this GO :0010950 and positive regulation of endopeptidase activity and biological process this GO :0017147 and Wnt-protein binding and molecular function this GO :0021915 and neural tube development and biological process this GO :0030111 and regulation of Wnt signaling pathway and biological process this GO :0030165 and PDZ domain binding and molecular function this GO :0030178 and negative regulation of Wnt signaling pathway and biological process this GO :0030199 and collagen fibril organization and biological process this GO :0030307 and positive regulation of cell growth and biological process this GO :0030308 and negative regulation of cell growth and biological process this GO :0030336 and negative regulation of cell migration and biological process this GO :0030514 and negative regulation of BMP signaling pathway and biological process this GO :0031012 and extracellular matrix and cellular component this GO :0031668 and cellular response to extracellular stimulus and biological process this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0033630 and positive regulation of cell adhesion mediated by integrin and biological process this GO :0035413 and positive regulation of catenin import into nucleus and biological process this GO :0036342 and post-anal tail morphogenesis and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042493 and response to drug and biological process this GO :0042662 and negative regulation of mesodermal cell fate specification and biological process this GO :0042733 and embryonic digit morphogenesis and biological process this GO :0042813 and Wnt-activated receptor activity and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043154 and negative regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0043508 and negative regulation of JUN kinase activity and biological process this GO :0045600 and positive regulation of fat cell differentiation and biological process this GO :0045669 and positive regulation of osteoblast differentiation and biological process this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0045892 and negative regulation of transcription, this GO :0001968 : fibronectin binding, this GO :0001968 : fibronectin binding and also this GO :0005178 : integrin binding and also this GO :0005515 : protein binding and also this GO :0010577 : metalloenzyme activator activity and also this GO :0017147 : Wnt-protein binding and also this GO :0030165 : PDZ domain binding and also this GO :0042813 : Wnt-activated receptor activity and also this GO :0048018 : receptor a this GO nist activity and also this GO :0061133 : endopeptidase activator activity, this GO :0005178 : integrin binding, this GO :0005515 : protein binding, this GO :0010577 : metalloenzyme activator activity, this GO :0017147 : Wnt-protein binding, this GO :0030165 : PDZ domain binding, this GO :0042813 : Wnt-activated receptor activity, this GO :0048018 : receptor a this GO nist activity, this GO :0061133 : endopeptidase activator activity, secreted frizzled-related protein 2 |
| Identity: |
10777 |
| Gene: |
SFRP2 |
More about : SFRP2 |
| Long gene name: |
secreted frizzled related protein 2 |
| Synonyms: |
SARP1 SDF-5 FRP-2 |
| Locus: |
4q31, 3 |
| Discovery year: |
1998-07-15 |
| GenBank acession: |
AF017986 |
| Entrez gene record: |
6423 |
| Pubmed identfication: |
9391078 |
| Classification: |
Secreted frizzled-related proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000161559 |