| RNA islotion kits: |
(RNAzol B, 100 ml, 1:72-74, 2, 2, 2, 3, 3, 3, 4, 4, 4, 4, 5, 5, 5, 6, 1982, 1987, 1991, A Rapid Method for Preparing DNA from Blood, Add 0, Add 800 ul of DNA STAT-60 reagent per 1 ml of RNAzol B or RNA STAT-60 used for the initial homogenization, Add at least 1 ml of 75% ethanol per 1 ml of the DNA STAT-60 used for the initial homogenization, An incubation for 10-15 minutes at 55-60oC may be required to dissolve DNA samples, At the end of the procedure, Avoid breathing vapor, B, BUT NOT SUPPLIED Chloroform (ACS grade) Isopropanol (ACS grade) Ethanol (ACS grade) 4, Biotechniques 8:148-149, CELLS: Cells grown in mono layer are lysed directly in a culture dish by adding DNA STAT-60 (1 ml/3, Can be fatal, Can be used to back extract DNA from ", Cells grown in suspension are sedimented then lysed in a DNA STAT-60 (1 ml per 5-10 x 106 cells by repetitive pipetting, Centrifuge the homogenate at 1000 g (min, Centrifuge the homogenate at 12, Chomczynski, DNA Extraction 1 vol, DNA Precipitation 0, DNA Wash 75% ethanol, DNA remains in the aqueous phase whereas RNA and proteins are in the interface and organic phase, DNA remains in the aqueous phase whereas RNA and proteins are in the interface and the organic phase, Dissolve the DNA pellet in water or in 1 mM EDTA, Do not get on skin or clothing, Does not contain phenol or hazardous organics, E, Following centrifugation the homogenate separates into two phases: a lower organic phase and the upper aqueous phase, Following centrifugation the homogenate separates into two phases: a lower organic phase and the upper aqueous phase, Following tissue or cell homogenization in the DNA STAT-60, Fritsch and J, G, Homogenization DNA STAT-60 (1 ml per 50-100 mg tissue, Isolates genomic DNA from samples of human, Maniatis, Molecular Cloning: A Laboratory Manual, P, PCR Methods Applic, PREPARATION Ready to use, PROTOCOL DNA isolation by the DNA STAT-60TM method includes the following steps: 1, Pass the pellet a few times through a pipette tip, Protect from exposure to light, Protocol can be completed in 60 minutes, REAGENTS REQUIRED, REAGENTS SUPPLIED DNA STAT-60TM 50 ml, RECOVERING DNA FROM ", REFERENCES 1, RNA STAT-60 PREPS), RNA STAT-60), RNA and proteins are sequestered in organic and interface: DNA remains in upper aqueous phase, RNA preps (i, RNAzol B, Read warning note on bottle, Refer to expiration date stamped on label, SPECIAL HANDLING PRECAUTIONS The DNA STAT-60 contains an irritant (Guanidinium Salts), STABILITY 6 months, STORAGE Refrigerate at 2-8oC, Sambrook, Single Step Method and RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction, Single monophasic reagent with extended shelf life, Suited for PCR Screening of Transgenes in Mice, T, TISSUES: Homogenate tissues in the DNA STAT-60 (1 ml/50-100 mg tissue) in a glass-Teflon or polytron homogenizer, The DNA STAT-60 isolates high molecular weight genomic DNA from samples of human, The DNA STAT-60 method does not require ultra centrifugation, The DNA remains in the aqueous phase while RNA and other cellular components, Unless stated otherwise the procedure is carried out at room temperature, When working with DNA STAT-60 use gloves and eye protection (shield, Winberg, a single monophasic reagent containing a chaotropic cell disrupter and a non-corrosive phenol free extraction reagent, add 0, and Sacchi, and after the addition of chloroform, and bacterial origin and is particularly well suited for the simultaneous processing of multiple samples, and can be completed in under 1 hour, animal, animal, are preferentially partitioned in the organic phase and interface, cover the sample tightly, dry the DNA pellet briefly by air drying (5-10 min, including proteins, labor intensive methods of genomic DNA isolation, of chloroform, of homogenate + 0, of isopropanol, or 200 ml bottle containing clear solution of DNA STAT-60 TM, or 5-10 x 106 cells), pH 7, plant, plant, replaces cumbersome, safety goggles), shake vigorously for 15 seconds and let it stay at room temperature for 2-3 minutes, shake vigorously for 15 seconds and let it stay at room temperature for 2-3 minutes, the homogenate separates into two phases: the aqueous phase and organic phase, yeast, yeast and bacterial origin, ), ) for 15 minutes at 4oC, ) for 15 minutes at 4oC, ) for 5 minutes at 4oC, )-12, 000 g (max, 000 g (max, 1 DNA REVERSE EXTRACTION Remove aqueous layer containing RNA, 1 HOMOGENIZATION A, 2 DNA EXTRACTION Following homogenization, 2 DNA PRECIPITATION Follow procedure as outlined in section 4, 2 ml of chloroform per 1 ml of DNA STAT-60, 2 ml of chloroform per 1 ml of DNA STAT-60, 2 vol, 3 DNA PRECIPITATION Remove supernatant and wash the DNA pellet once with 75% ethanol by vortexing and subsequent centrifugation at 7, 4, 5 cm petri dish) and passing cell lysate 5-10 times through a pipette, 5 vol, 500 g (max, DNA STAT-60, SINGLE STEP METHOD", SINGLE STEP METHOD", T, e |