| Antigen detectable with this ELISA kit: |
Retinoblastoma Protein 1 (RB1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
054ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoblastoma Protein 1 (RB1), The concentration of Retinoblastoma Protein 1 (RB1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoblastoma Protein 1 (RB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Retinoblastoma Protein 1 (RB1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Retinoblastoma Protein 1 (RB1) |
| Short name: |
Retinoblastoma Protein 1 (RB1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Retinoblastoma Protein 1 (retinoblastoma 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, DNA-templated and biological process this GO :0006469 and negative regulation of protein kinase activity and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007070 and negative regulation of transcription from RNA polymerase II promoter during mitosis and biological process this GO :0007093 and mitotic cell cycle checkpoint and biological process this GO :0007265 and Ras protein signal transduction and biological process this GO :0007346 and regulation of mitotic cell cycle and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008150 and biological process and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0016032 and viral process and biological process this GO :0016514 and SWI/SNF complex and cellular component this GO :0016605 and PML body and cellular component this GO :0019899 and enzyme binding and molecular function this GO :0019900 and kinase binding and molecular function this GO :0030182 and neuron differentiation and biological process this GO :0030521 and androgen receptor signaling pathway and biological process this GO :0031134 and sister chromatid biorientation and biological process this GO :0031175 and neuron projection development and biological process this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0034088 and maintenance of mitotic sister chromatid cohesion and biological process this GO :0034349 and glial cell apoptotic process and biological process this GO :0035189 and Rb-E2F complex and cellular component this GO :0035914 and skeletal muscle cell differentiation and biological process this GO :0042551 and neuron maturation and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043353 and enucleate erythrocyte differentiation and biological process this GO :0043433 and negative regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0043550 and regulation of lipid kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045445 and myoblast differentiation and biological process this GO :0045651 and positive regulation of macrophage differentiation and biological process this GO :0045786 and negative regulation of cell cycle and biological process this GO :0045842 and positive regulation of mitotic metaphase/anaphase transition and biological process this GO :0045879 and negative regulation of smoothened signaling pathway and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048565 and digestive tract development and biological process this GO :0048667 and cell morphogenesis involved in neuron differentiation and biological process this GO :0050680 and negative regulation of epithelial cell proliferation and biological process this GO :0050681 and androgen receptor binding and molecular function this GO :0051146 and striated muscle cell differentiation and biological process this GO :0051219 and phosphoprotein binding and molecular function this GO :0051301 and cell division and biological process this GO :0051402 and neuron apoptotic process and biological process this GO :0051726 and regulation of cell cycle and biological process this GO :0071459 and protein localization to chromosome, OSRC and p105-Rb and pp110 and PPP1R130 and pRb and RB, RB1 and IDBG-32092 and ENSG00000139687 and 5925, Rb1 and IDBG-179829 and ENSMUSG00000022105 and 19645, centromeric region and biological process this GO :0071922 and regulation of cohesin localization to chromatin and biological process this GO :0071930 and negative regulation of transcription involved in G1/S transition of mitotic cell cycle and biological process this GO :0090230 and regulation of centromere complex assembly and biological process this GO :0097284 and hepatocyte apoptotic process and biological process this GO :2000134 and negative regulation of G1/S transition of mitotic cell cycle and biological process, nuclei, phosphoprotein binding, this GO :0000075 and cell cycle checkpoint and biological process this GO :0000082 and G1/S transition of mitotic cell cycle and biological process this GO :0000083 and regulation of transcription involved in G1/S transition of mitotic cell cycle and biological process this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000278 and mitotic cell cycle and biological process this GO :0000785 and chromatin and cellular component this GO :0001047 and core promoter binding and molecular function this GO :0001102 and RNA polymerase II activating transcription factor binding and molecular function this GO :0003674 and molecular function and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003713 and transcription coactivator activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005575 and cellular component and cellular component this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005819 and spindle and cellular component this GO :0006338 and chromatin remodeling and biological process this GO :0006351 and transcription, this GO :0001047 : core promoter binding, this GO :0001047 : core promoter binding and also this GO :0001102 : RNA polymerase II activating transcription factor binding and also this GO :0003674 : molecular_function and also this GO :0003677 : DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003713 : transcription coactivator activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0019899 : enzyme binding and also this GO :0019900 : kinase binding and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0042802 : identical protein binding and also this GO :0050681 : androgen receptor binding and also this GO :0051219 : phosphoprotein binding, this GO :0001102 : RNA polymerase II activating transcription factor binding, this GO :0003674 : molecular_function, this GO :0003677 : DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003713 : transcription coactivator activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0019899 : enzyme binding, this GO :0019900 : kinase binding, this GO :0031625 : ubiquitin protein ligase binding, this GO :0042802 : identical protein binding, this GO :0050681 : androgen receptor binding, this GO :0051219 : phosphoprotein binding, 68871 and IDBG-629082 and ENSBTAG00000006640 and 534712, retinoblastoma 1 |
| Identity: |
9884 |
| Gene: |
RB1 |
More about : RB1 |
| Long gene name: |
RB transcriptional corepressor 1 |
| Synonyms gene: |
OSRC |
| Synonyms gene name: |
osteosarcoma retinoblastoma 1 |
| Synonyms: |
RB PPP1R130 |
| Synonyms name: |
prepro-retinoblastoma-associated protein protein phosphatase 1, regulatory subunit 130 |
| Locus: |
13q14, 2 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
M15400 |
| Entrez gene record: |
5925 |
| Pubmed identfication: |
1857421 15057823 |
| RefSeq identity: |
NM_000321 |
| Classification: |
Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000016900 |
| Locus Specific Databases: |
RB1-LSDB LRG_517 |