| Antigen detectable with this ELISA kit: |
G Protein Coupled 12 (P2RY12), Purinergic Receptor P2Y |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
061ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, G Protein Coupled 12 (P2RY12), G Protein Coupled 12 (P2RY12), G Protein Coupled 12 (P2RY12), G Protein Coupled 12 (P2RY12) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Purinergic Receptor P2Y, The concentration of Purinergic Receptor P2Y, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Purinergic Receptor P2Y, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Purinergic Receptor P2Y, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
G Protein Coupled 12 (P2RY12), Purinergic Receptor P2Y |
| Short name: |
G Protein Coupled 12 (P2RY12) ELISA kit, Purinergic Receptor P2Y |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
12) Enzyme-linked immunosorbent assay reagent, G Protein Coupled 12 (purinergic receptor P2Y, G-protein coupled, Purinergic Receptor P2Y |
| Alternative technique: |
elisas |
| Alternative to gene target: |
12, Cell surfaces, G-protein coupled, P2RY12 and IDBG-61579 and ENSG00000169313 and 64805, P2RY12 and IDBG-637609 and ENSBTAG00000015837 and 408007, P2ry12 and IDBG-147152 and ENSMUSG00000036353 and 70839, guanyl-nucleotide exchange factor activity, this GO :0001609 and G-protein coupled adenosine receptor activity and molecular function this GO :0001621 and ADP receptor activity and molecular function this GO :0001973 and adenosine receptor signaling pathway and biological process this GO :0004930 and G-protein coupled receptor activity and molecular function this GO :0005085 and guanyl-nucleotide exchange factor activity and molecular function this GO :0005739 and mitochondrion and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005901 and caveola and cellular component this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007188 and adenylate cyclase-modulating G-protein coupled receptor signaling pathway and biological process this GO :0007193 and adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway and biological process this GO :0007596 and blood coagulation and biological process this GO :0007599 and hemostasis and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009925 and basal plasma membrane and cellular component this GO :0010700 and negative regulation of norepinephrine secretion and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0030030 and cell projection organization and biological process this GO :0030168 and platelet activation and biological process this GO :0031224 and intrinsic component of membrane and cellular component this GO :0035589 and G-protein coupled purinergic nucleotide receptor signaling pathway and biological process this GO :0043270 and positive regulation of ion transport and biological process this GO :0043491 and protein kinase B signaling and biological process this GO :0043547 and positive regulation of GTPase activity and biological process this GO :0045596 and negative regulation of cell differentiation and biological process this GO :0051924 and regulation of calcium ion transport and biological process this GO :0070527 and platelet aggregation and biological process this GO :0070588 and calcium ion transmembrane transport and biological process this GO :0071805 and potassium ion transmembrane transport and biological process, this GO :0001609 : G-protein coupled adenosine receptor activity, this GO :0001609 : G-protein coupled adenosine receptor activity and also this GO :0001621 : ADP receptor activity and also this GO :0004930 : G-protein coupled receptor activity and also this GO :0005085 : guanyl-nucleotide exchange factor activity, this GO :0001621 : ADP receptor activity, this GO :0004930 : G-protein coupled receptor activity, this GO :0005085 : guanyl-nucleotide exchange factor activity, purinergic receptor P2Y |
| Identity: |
18124 |
| Gene: |
P2RY12 |
More about : P2RY12 |
| Long gene name: |
purinergic receptor P2Y12 |
| Synonyms gene name: |
12 , G-protein coupled, purinergic receptor P2Y |
| Synonyms: |
P2Y12 SP1999 HORK3 |
| Locus: |
3q25, 1 |
| Discovery year: |
2002-12-10 |
| GenBank acession: |
AJ320495 |
| Pubmed identfication: |
11502873 11104774 |
| Classification: |
Purinergic receptors P2Y |
| Havana BLAST/BLAT: |
OTTHUMG00000159863 |
| Locus Specific Databases: |
LRG_569 |